A novel stepwise recovery strategy of cellulase adsorbed to the residual substrate after hydrolysis of steam exploded wheat straw
文献类型：期刊论文
| 作者 | Xu, Jian; Chen, Hongzhang
|
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2007-10-01 |
| 卷号 | 143期号:1页码:93-100 |
| 关键词 | cellulase enzymatic hydrolysis residual substrate stepwise recovery strategy specific activity |
| ISSN号 | 0273-2289 |
| 其他题名 | Appl. Biochem. Biotechnol. |
| 中文摘要 | Cellulase distribution between residual substrate and supernatant in the process of enzymatic hydrolysis of steam-exploded wheat straw was investigated. Subsequently, a novel stepwise recovery strategy with three successive steps was adopted to recover cellulase adsorbed to the residual substrate. The results showed that cellulase protein in the supernatant increased as the hydrolysis time increased. When hydrolysis ended, the cellulase remaining on the residual substrate accounted for 33-42% of the original added cellulase according to the different cellulase loading. To obtain the maximum cellulase recovery rate, the residual substrate was dealt with in three successive steps: washed with sodium acetate buffer ( step 1), shaken with sodium acetate buffer ( step 2), and then treated with 0.0015 mol/L, pH 10 Ca(OH)(2) ( step 3). The total cellulase protein recovered by the three steps reached 96.70-98.14%. The enzyme activity of cellulase recovered by the first two steps was kept well. The ratios of the specific activity between the recovered cellulase and the original were 89-96%, which was by far higher than that using step 3 ( the value was 48%similar to 56%). |
| 英文摘要 | Cellulase distribution between residual substrate and supernatant in the process of enzymatic hydrolysis of steam-exploded wheat straw was investigated. Subsequently, a novel stepwise recovery strategy with three successive steps was adopted to recover cellulase adsorbed to the residual substrate. The results showed that cellulase protein in the supernatant increased as the hydrolysis time increased. When hydrolysis ended, the cellulase remaining on the residual substrate accounted for 33-42% of the original added cellulase according to the different cellulase loading. To obtain the maximum cellulase recovery rate, the residual substrate was dealt with in three successive steps: washed with sodium acetate buffer ( step 1), shaken with sodium acetate buffer ( step 2), and then treated with 0.0015 mol/L, pH 10 Ca(OH)(2) ( step 3). The total cellulase protein recovered by the three steps reached 96.70-98.14%. The enzyme activity of cellulase recovered by the first two steps was kept well. The ratios of the specific activity between the recovered cellulase and the original were 89-96%, which was by far higher than that using step 3 ( the value was 48%similar to 56%). |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | ENZYMATIC-HYDROLYSIS ; TRICHODERMA-REESEI ; ENZYMES ; WOOD |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000250070200008 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3486]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.SUNY Syracuse, Syracuse, NY 13210 USA |
| 推荐引用方式 GB/T 7714 | Xu, Jian,Chen, Hongzhang. A novel stepwise recovery strategy of cellulase adsorbed to the residual substrate after hydrolysis of steam exploded wheat straw[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2007,143(1):93-100. |
| APA | Xu, Jian,&Chen, Hongzhang.(2007).A novel stepwise recovery strategy of cellulase adsorbed to the residual substrate after hydrolysis of steam exploded wheat straw.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,143(1),93-100. |
| MLA | Xu, Jian,et al."A novel stepwise recovery strategy of cellulase adsorbed to the residual substrate after hydrolysis of steam exploded wheat straw".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 143.1(2007):93-100. |
入库方式： OAI收割
来源：过程工程研究所
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