Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2
文献类型：期刊论文
| 作者 | Xu, Jinxin; Jiang, Xin; Zhang, Yulong; Dong, Yan; Ma, Changli; Jiang, Hanqiu ; Zuo, Taisen; Chen, Rui; Ke, Yubin; Cheng, He
|
| 刊名 | COMMUNICATIONS BIOLOGY
 |
| 出版日期 | 2022 |
| 卷号 | 5期号:1页码:925 |
| DOI | 10.1038/s42003-022-03892-x |
| 文献子类 | Article |
| 英文摘要 | RNA replication and transcription machinery is an important drug target for fighting against coronavirus. Non-structure protein nsp8 was proposed harboring primase activity. However, the RNA primer synthesis mechanism of nsp8 is still largely unknown. Here, we purified dimer and tetramer forms of SARS-CoV-2 nsp8. Combined with dynamic light scattering, small-angle neutron scattering and thermo-stability analysis, we found that both dimer and tetramer become loosened and destabilized with decreasing salt concentration, and the dimer form is more stable than the tetramer form. Further investigation showed that nsp8 dimer and tetramer can undergo phase separation but exhibit different phase separation behaviors. Nsp8 dimer can form liquid-like droplets in the buffer with a low concentration of NaCl; phase separation of nsp8 tetramer depends on the assistance of RNA. Our findings on different phase separation behaviors of nsp8 dimer and tetramer may provide insight into the functional studies of nsp8 in coronavirus. |
| 电子版国际标准刊号 | 2399-3642 |
| 语种 | 英语 |
| WOS记录号 | WOS:000852533200003 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/299415]  |
| 专题 | 高能物理研究所_东莞分部 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Xu, Jinxin,Jiang, Xin,Zhang, Yulong,et al. Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2[J]. COMMUNICATIONS BIOLOGY,2022,5(1):925. |
| APA | Xu, Jinxin.,Jiang, Xin.,Zhang, Yulong.,Dong, Yan.,Ma, Changli.,...&Liu, Jinsong.(2022).Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2.COMMUNICATIONS BIOLOGY,5(1),925. |
| MLA | Xu, Jinxin,et al."Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2".COMMUNICATIONS BIOLOGY 5.1(2022):925. |
入库方式： OAI收割
来源：高能物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。