Facile purification of mono-PEGylated interleukin-1 receptor antagonist and its characterization with multi-angle laser light scattering
文献类型：期刊论文
| 作者 | Yu, Pengzhan; Zhang, Guicai; Bi, Jingxiu; Lu, Xiuling; Wang, Yunshan; Su, Zhiguo |
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2007-06-01 |
| 卷号 | 42期号:6页码:971-977 |
| 关键词 | cytokine PEGylation recombinant human interleukin-1 receptor antagonist multi-angle laser light scattering bioactivity |
| ISSN号 | 1359-5113 |
| 其他题名 | Process Biochem. |
| 中文摘要 | Recombinant human interleukin-1 receptor antagonist (rhIL-1ra) was chemically conjugated with succinimidyl carbonate monomethoxyl polyethylene glycols of 5 kDa (SC-PEG(5k)) and 10 kDa (SC-PEG(10k)) molecular weight. A facile purification of the conjugates was achieved by one-step cationic exchange chromatography. The purity of mono-PEGylated protein was greater than 95%. The purified conjugate was characterized by multi-angle laser light scattering (MALLS) for determining the apparent gyration radius (r(g)) and hydrodynamic radius (rh). MALLS results showed that the conjugation of PEG markedly enhanced r(g) and r(h) of parent protein (r(g): from 15.7 to 48.2 nm for the PEG(5k) and 81.9 nm for the PEG(10k); r(h): from 4.2 to 5 8.4 nm for the PEG(5k) and 102.3 nm for the PEG 100. The PEGylated rhIL-1ra retained 44.6% of binding activities to the cell receptor for PEG(5k) and 40.2% for PEG(10k), compared to the original protein. (C) 2007 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Recombinant human interleukin-1 receptor antagonist (rhIL-1ra) was chemically conjugated with succinimidyl carbonate monomethoxyl polyethylene glycols of 5 kDa (SC-PEG(5k)) and 10 kDa (SC-PEG(10k)) molecular weight. A facile purification of the conjugates was achieved by one-step cationic exchange chromatography. The purity of mono-PEGylated protein was greater than 95%. The purified conjugate was characterized by multi-angle laser light scattering (MALLS) for determining the apparent gyration radius (r(g)) and hydrodynamic radius (rh). MALLS results showed that the conjugation of PEG markedly enhanced r(g) and r(h) of parent protein (r(g): from 15.7 to 48.2 nm for the PEG(5k) and 81.9 nm for the PEG(10k); r(h): from 4.2 to 5 8.4 nm for the PEG(5k) and 102.3 nm for the PEG 100. The PEGylated rhIL-1ra retained 44.6% of binding activities to the cell receptor for PEG(5k) and 40.2% for PEG(10k), compared to the original protein. (C) 2007 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | RHEUMATOID-ARTHRITIS ; POLYETHYLENE-GLYCOL ; HEPATITIS-C ; PROTEIN PEGYLATION ; PEPTIDE ; PHARMACEUTICALS ; CHROMATOGRAPHY ; ANAKINRA ; EFFICACY ; BINDING |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000247084000006 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3498]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 3.Beijing Baiao Pharmaceut Co Ltd, Beijing 102200, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Pengzhan,Zhang, Guicai,Bi, Jingxiu,et al. Facile purification of mono-PEGylated interleukin-1 receptor antagonist and its characterization with multi-angle laser light scattering[J]. PROCESS BIOCHEMISTRY,2007,42(6):971-977. |
| APA | Yu, Pengzhan,Zhang, Guicai,Bi, Jingxiu,Lu, Xiuling,Wang, Yunshan,&Su, Zhiguo.(2007).Facile purification of mono-PEGylated interleukin-1 receptor antagonist and its characterization with multi-angle laser light scattering.PROCESS BIOCHEMISTRY,42(6),971-977. |
| MLA | Yu, Pengzhan,et al."Facile purification of mono-PEGylated interleukin-1 receptor antagonist and its characterization with multi-angle laser light scattering".PROCESS BIOCHEMISTRY 42.6(2007):971-977. |
入库方式： OAI收割
来源：过程工程研究所
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