Investigation on PEGylation strategy of recombinant human interleukin-1 receptor antagonist
文献类型：期刊论文
| 作者 | Yu, Pengzhan; Zheng, Chunyang; Chen, Jing; Zhang, Guifeng; Liu, Yongdong; Suo, Xiaoyan; Zhang, Guicai; Su, Zhiguo |
| 刊名 | BIOORGANIC & MEDICINAL CHEMISTRY
 |
| 出版日期 | 2007-08-15 |
| 卷号 | 15期号:16页码:5396-5405 |
| 关键词 | interleukin-1 receptor antagonist PEGylation binding activities to the receptors |
| ISSN号 | 0968-0896 |
| 其他题名 | Bioorg. Med. Chem. |
| 中文摘要 | Although PEGylation is a potential approach to prolong the half-lives and reduce the dosing frequency of therapeutic proteins, conjugation behaviors of polymer have pivotal effects on the remaining bioactivities of the derivatives. In this study, the PEGylation strategy of recombinant human interleukin-1 receptor antagonist was investigated. The random conjugation of polyethylene glycol to amino groups on the protein resulted in a severe loss of activity and only retained 9.8% of the activity. In contrast, the PEGylation at the thiol groups had moderate effects on the bioactivity of protein and 40% of activity was conserved. The results suggested that the thiol-target PEGylation was more beneficial for IL-1ra. (c) 2007 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Although PEGylation is a potential approach to prolong the half-lives and reduce the dosing frequency of therapeutic proteins, conjugation behaviors of polymer have pivotal effects on the remaining bioactivities of the derivatives. In this study, the PEGylation strategy of recombinant human interleukin-1 receptor antagonist was investigated. The random conjugation of polyethylene glycol to amino groups on the protein resulted in a severe loss of activity and only retained 9.8% of the activity. In contrast, the PEGylation at the thiol groups had moderate effects on the bioactivity of protein and 40% of activity was conserved. The results suggested that the thiol-target PEGylation was more beneficial for IL-1ra. (c) 2007 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Medicinal ; Chemistry, Organic |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Chemistry |
| 关键词[WOS] | SITE-SPECIFIC MUTAGENESIS ; CHRONIC HEPATITIS-C ; POLYETHYLENE-GLYCOL ; DRUG-DELIVERY ; RHEUMATOID-ARTHRITIS ; PROTEIN MODIFICATION ; DOUBLE-BLIND ; ASSAY ; PHARMACEUTICALS ; IDENTIFICATION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000248172700003 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3499]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 3.Beijing Baiao Pharmaceut Co Ltd, Beijing 102200, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Pengzhan,Zheng, Chunyang,Chen, Jing,et al. Investigation on PEGylation strategy of recombinant human interleukin-1 receptor antagonist[J]. BIOORGANIC & MEDICINAL CHEMISTRY,2007,15(16):5396-5405. |
| APA | Yu, Pengzhan.,Zheng, Chunyang.,Chen, Jing.,Zhang, Guifeng.,Liu, Yongdong.,...&Su, Zhiguo.(2007).Investigation on PEGylation strategy of recombinant human interleukin-1 receptor antagonist.BIOORGANIC & MEDICINAL CHEMISTRY,15(16),5396-5405. |
| MLA | Yu, Pengzhan,et al."Investigation on PEGylation strategy of recombinant human interleukin-1 receptor antagonist".BIOORGANIC & MEDICINAL CHEMISTRY 15.16(2007):5396-5405. |
入库方式： OAI收割
来源：过程工程研究所
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