Preferential Regulation of Transient Protein-Protein Interaction by the Macromolecular Crowders
文献类型：期刊论文
| 作者 | Gong, Z; Yang, J ; Qin, LY; Tang, C; Jiang, HQ; Ke, YB; Dong, X
|
| 刊名 | Journal of Physical Chemistry B
 |
| 出版日期 | 2022 |
| 卷号 | 126期号:26页码:4840-4848 |
| ISSN号 | 15206106 |
| DOI | 10.1021/acs.jpcb.2c02713 |
| 文献子类 | Article |
| 英文摘要 | The environmental condition is a critical regulation factor for protein behavior in solution. Several studies have shown that macromolecular crowders can modulate protein structures, interactions, and functions. Recent publications described the regulation of specific interaction by macromolecular crowders. However, the other category of protein-protein interaction, namely, the transient interaction, is rarely investigated, especially from the perspective of protein structure to study transient interactions between proteins. Here, we used nuclear magnetic resonance and small-angle X-ray/neutron scattering methods to structurally investigate the ensemble of the protein complex in dilute buffer and crowded environments. Histidine phosphocarrier protein (HPr) and the N-terminal domain of enzyme I (EIN) are the important components of the bacterial phosphotransfer system. Our results show that the addition of Ficoll-70 promotes HPr molecules to form the encounter complex with EIN maintained by long-range electrostatic interaction. However, when macromolecular crowder BSA is used, the soft interaction between BSA and HPr perturbs the active site of HPr, driving HPr to form an encounter complex with EIN at the weakly charged interface. Our results indicate that different macromolecular crowders could influence transient EIN-HPr interaction through different mechanisms and provide new insights into protein-protein interaction regulation in native environments. © 2022 American Chemical Society. All rights reserved. |
| 电子版国际标准刊号 | 15205207 |
| 语种 | 英语 |
| WOS记录号 | WOS:000819994800001 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/299758]  |
| 专题 | 高能物理研究所_东莞分部 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Gong, Z,Yang, J,Qin, LY,et al. Preferential Regulation of Transient Protein-Protein Interaction by the Macromolecular Crowders[J]. Journal of Physical Chemistry B,2022,126(26):4840-4848. |
| APA | Gong, Z.,Yang, J.,Qin, LY.,Tang, C.,Jiang, HQ.,...&Dong, X.(2022).Preferential Regulation of Transient Protein-Protein Interaction by the Macromolecular Crowders.Journal of Physical Chemistry B,126(26),4840-4848. |
| MLA | Gong, Z,et al."Preferential Regulation of Transient Protein-Protein Interaction by the Macromolecular Crowders".Journal of Physical Chemistry B 126.26(2022):4840-4848. |
入库方式： OAI收割
来源：高能物理研究所
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