Polyethylene glycol improves conjugation of bovine hemoglobin and human serum albumin in a controlled ratio
文献类型：期刊论文
| 作者 | Zheng, Chunyang; Bi, Jingxiu; Ma, Guanghui; Su, Zhiguo |
| 刊名 | ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
 |
| 出版日期 | 2007 |
| 卷号 | 35期号:6页码:568-584 |
| 关键词 | blood substitute bovine hemoglobin glutaraldehyde human serum albumin polyethylene glycol |
| ISSN号 | 1073-1199 |
| 其他题名 | Artif. Cells Blood Substit. Biotechnol. |
| 中文摘要 | Direct conjugation of bovine hemoglobin (Hb) and human serum albumin (HSA) with glutaraldehyde would result in a complex mixture of dimers Hb-Hb, Hb-HSA, HSA-HSA and other oligomers. To obtain a high yield of target Hb-HSA, modulation of the reaction environment was carried out. It was found that polyethylene glycol (PEG), a hydrophilic polymer, could improve the yield of Hb-HSA conjugate. The degree of improvement depended on the molecular weight and concentration of PEG. Under optimum condition of 9% (w/v) of PEG 4000, the reaction proceeded in a controlled mode with conversion yield of starting proteins to Hb-HSA increasing from 6% to 30%. The purity was about 88% of the total conjugates. Furthermore, the impurities were mainly tetrameric molecules of two Hb-HSA conjugates. The improvement could be attributed to the "microcompartment" created by addition of polyethylene glycol, which brings HSA and Hb close together, thus increasing the chance of conjugation between the two molecules. |
| 英文摘要 | Direct conjugation of bovine hemoglobin (Hb) and human serum albumin (HSA) with glutaraldehyde would result in a complex mixture of dimers Hb-Hb, Hb-HSA, HSA-HSA and other oligomers. To obtain a high yield of target Hb-HSA, modulation of the reaction environment was carried out. It was found that polyethylene glycol (PEG), a hydrophilic polymer, could improve the yield of Hb-HSA conjugate. The degree of improvement depended on the molecular weight and concentration of PEG. Under optimum condition of 9% (w/v) of PEG 4000, the reaction proceeded in a controlled mode with conversion yield of starting proteins to Hb-HSA increasing from 6% to 30%. The purity was about 88% of the total conjugates. Furthermore, the impurities were mainly tetrameric molecules of two Hb-HSA conjugates. The improvement could be attributed to the "microcompartment" created by addition of polyethylene glycol, which brings HSA and Hb close together, thus increasing the chance of conjugation between the two molecules. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering ; Materials Science |
| 关键词[WOS] | PURIFICATION ; BLOOD ; CHROMATOGRAPHY ; PROTEIN |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000251751100003 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3529]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 10049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Chunyang,Bi, Jingxiu,Ma, Guanghui,et al. Polyethylene glycol improves conjugation of bovine hemoglobin and human serum albumin in a controlled ratio[J]. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,2007,35(6):568-584. |
| APA | Zheng, Chunyang,Bi, Jingxiu,Ma, Guanghui,&Su, Zhiguo.(2007).Polyethylene glycol improves conjugation of bovine hemoglobin and human serum albumin in a controlled ratio.ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,35(6),568-584. |
| MLA | Zheng, Chunyang,et al."Polyethylene glycol improves conjugation of bovine hemoglobin and human serum albumin in a controlled ratio".ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY 35.6(2007):568-584. |
入库方式： OAI收割
来源：过程工程研究所
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