The role of pH and its control on effective conjugation of bovine hemoglobin and human serum albumin
文献类型：期刊论文
| 作者 | Zheng, Chunyang; Ma, Guanghui; Su, Zhiguo |
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2007-03-01 |
| 卷号 | 42期号:3页码:303-309 |
| 关键词 | human serum albumin (HSA) bovine hemoglobin (Hb) borax-borate buffer-mannite system conjugate blood substitute |
| ISSN号 | 1359-5113 |
| 其他题名 | Process Biochem. |
| 中文摘要 | Human serum albumin (HSA) and bovine hemoglobin (Hb) conjugate is a promising candidate as a blood substitute. However, preparation of the conjugate is problematic because both proteins tend to conjugate between themselves rather than crosslink each other. In this work, a facile process for conjugation of Hb and HSA was developed through control strategy of the reaction. The reaction was carried out in a buffer containing borax-borate and mannite. The borax-borate was used for pH buffering while mannite was used as a pH switch and a reaction promoter. As a result, self-conjugation of Hb and self-conjugation of HSA were minimized. After the one-step conjugation reaction in aqueous solution, followed by the one-step purification by ion-exchange chromatography, the conjugate of HSA and Hb was obtained with the total yield about 50%. The P-50 and the Hill coefficient for the product were 16.1 mmHg and 1.82, respectively. (c) 2006 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Human serum albumin (HSA) and bovine hemoglobin (Hb) conjugate is a promising candidate as a blood substitute. However, preparation of the conjugate is problematic because both proteins tend to conjugate between themselves rather than crosslink each other. In this work, a facile process for conjugation of Hb and HSA was developed through control strategy of the reaction. The reaction was carried out in a buffer containing borax-borate and mannite. The borax-borate was used for pH buffering while mannite was used as a pH switch and a reaction promoter. As a result, self-conjugation of Hb and self-conjugation of HSA were minimized. After the one-step conjugation reaction in aqueous solution, followed by the one-step purification by ion-exchange chromatography, the conjugate of HSA and Hb was obtained with the total yield about 50%. The P-50 and the Hill coefficient for the product were 16.1 mmHg and 1.82, respectively. (c) 2006 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | TRANSFUSION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000245021300001 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3531]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Chunyang,Ma, Guanghui,Su, Zhiguo. The role of pH and its control on effective conjugation of bovine hemoglobin and human serum albumin[J]. PROCESS BIOCHEMISTRY,2007,42(3):303-309. |
| APA | Zheng, Chunyang,Ma, Guanghui,&Su, Zhiguo.(2007).The role of pH and its control on effective conjugation of bovine hemoglobin and human serum albumin.PROCESS BIOCHEMISTRY,42(3),303-309. |
| MLA | Zheng, Chunyang,et al."The role of pH and its control on effective conjugation of bovine hemoglobin and human serum albumin".PROCESS BIOCHEMISTRY 42.3(2007):303-309. |
入库方式： OAI收割
来源：过程工程研究所
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