A macrokinetic model for myeloma cell culture based on stoichiometric balance
文献类型：期刊论文
| 作者 | Zhou, Feng; Bi, Jingxiu; Zeng, An-ping; Yuan, Jingqi |
| 刊名 | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
 |
| 出版日期 | 2007-02-01 |
| 卷号 | 46期号:46页码:85-95 |
| 关键词 | macrokinetic model metabolic pathways metabolic shift myeloma cell culture stoichiometric model |
| ISSN号 | 0885-4513 |
| 其他题名 | Biotechnol. Appl. Biochem. |
| 中文摘要 | A macrokinetic model for a myeloma cell line is proposed. The model describes the dynamic balances of lactate, alanine, ATP and Ill during the metabolsim of glucose, glutamine and other amino acids. The metabolic pathways mainly include glycolysis, glutaminolysis, the trcicarboxylic acid cycle, the formation and utilization of amino acids, the respiratory chain, cell growth and cell death. The metabolic shift of glucose is especially considered because of a change in the rate of glycolysis. Thus the model functions in three modes to describe the behaviour of the myeloma cell line. On the basis of this model the macrokinetic bioreaction rates such as the specific substrate consumption rate, the specific growth rate, the specific acetylCoA formation rate, as well as the specific oxygen uptake rate, are estimated. The specific substrate consumption rate and the specific growth rate are then coupled into a bioreactor model such that the key variables, i.e., the cell density, the substrate and metabolite concentrations, are obtained. Experiments with batch and fed-batch cultures of a myeloma cell line (X63-Ag8.653) were used to validate the model. The prediction of the model was simulated by the rolling prediction approach. |
| 英文摘要 | A macrokinetic model for a myeloma cell line is proposed. The model describes the dynamic balances of lactate, alanine, ATP and Ill during the metabolsim of glucose, glutamine and other amino acids. The metabolic pathways mainly include glycolysis, glutaminolysis, the trcicarboxylic acid cycle, the formation and utilization of amino acids, the respiratory chain, cell growth and cell death. The metabolic shift of glucose is especially considered because of a change in the rate of glycolysis. Thus the model functions in three modes to describe the behaviour of the myeloma cell line. On the basis of this model the macrokinetic bioreaction rates such as the specific substrate consumption rate, the specific growth rate, the specific acetylCoA formation rate, as well as the specific oxygen uptake rate, are estimated. The specific substrate consumption rate and the specific growth rate are then coupled into a bioreactor model such that the key variables, i.e., the cell density, the substrate and metabolite concentrations, are obtained. Experiments with batch and fed-batch cultures of a myeloma cell line (X63-Ag8.653) were used to validate the model. The prediction of the model was simulated by the rolling prediction approach. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | INTRACELLULAR FLUX ANALYSIS ; STEADY-STATE MULTIPLICITY ; HYBRIDOMA CELLS ; ANTIBODY-PRODUCTION ; DYNAMIC-MODEL ; STRUCTURED MODEL ; MURINE HYBRIDOMA ; REACTION NETWORK ; MAMMALIAN-CELLS ; MASS BALANCES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000244194600002 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3533]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Dept Automat, Shanghai 200240, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing 200080, Peoples R China 3.German Res Ctr Biotechnol, Div Biochem Engn, D-38124 Braunschweig, Germany |
| 推荐引用方式 GB/T 7714 | Zhou, Feng,Bi, Jingxiu,Zeng, An-ping,et al. A macrokinetic model for myeloma cell culture based on stoichiometric balance[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2007,46(46):85-95. |
| APA | Zhou, Feng,Bi, Jingxiu,Zeng, An-ping,&Yuan, Jingqi.(2007).A macrokinetic model for myeloma cell culture based on stoichiometric balance.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,46(46),85-95. |
| MLA | Zhou, Feng,et al."A macrokinetic model for myeloma cell culture based on stoichiometric balance".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 46.46(2007):85-95. |
入库方式： OAI收割
来源：过程工程研究所
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