Preparation of uniform-sized agarose beads by microporous membrane emulsification technique
文献类型：期刊论文
| 作者 | Zhou, Qing-Zhu; Wang, Lian-Yan; Ma, Guang-Hui; Su, Zhi-Guo |
| 刊名 | JOURNAL OF COLLOID AND INTERFACE SCIENCE
 |
| 出版日期 | 2007-07-01 |
| 卷号 | 311期号:1页码:118-127 |
| 关键词 | agarose beads membrane emulsification uniform-sized |
| ISSN号 | 0021-9797 |
| 其他题名 | J. Colloid Interface Sci. |
| 中文摘要 | Uniform-sized agarose beads were prepared by membrane emulsification technique in this study. Agarose was dissolved in boiling water (containing 0.9% sodium chloride) and used as water phase. A mixture of liquid paraffin and petroleum ether containing 4 wt% of hexaglycerin penta ester (PO-500) emulsifier was used as oil phase. At 55 degrees C, the water phase permeated through uniform pores of microporous membrane into the oil phase by a pressure of nitrogen gas to form uniform W/O emulsion. Then the emulsion was cooled down to room temperature under gentle agitation to form gel beads. The effect of oil phase, emulsifier, especially temperature on the uniformity of the beads were investigated and interpreted from interfacial tension between water phase and oil phase. Under optimized condition, the coefficient variation (C.V.) showing the size distribution of the beads was under 15%. This was the first report to prepare uniform agarose beads by membrane emulsification, and to investigate the effect of temperature on the size distribution of the droplets and beads. The beads with different size can be prepared by using membranes with different pore size, and the result showed that there was a linear relationship between the average diameter of beads and pore size of the membranes; beads with diameter from 15 to 60 pro were able to obtain in this study. (C) 2007 Elsevier Inc. All rights reserved. |
| 英文摘要 | Uniform-sized agarose beads were prepared by membrane emulsification technique in this study. Agarose was dissolved in boiling water (containing 0.9% sodium chloride) and used as water phase. A mixture of liquid paraffin and petroleum ether containing 4 wt% of hexaglycerin penta ester (PO-500) emulsifier was used as oil phase. At 55 degrees C, the water phase permeated through uniform pores of microporous membrane into the oil phase by a pressure of nitrogen gas to form uniform W/O emulsion. Then the emulsion was cooled down to room temperature under gentle agitation to form gel beads. The effect of oil phase, emulsifier, especially temperature on the uniformity of the beads were investigated and interpreted from interfacial tension between water phase and oil phase. Under optimized condition, the coefficient variation (C.V.) showing the size distribution of the beads was under 15%. This was the first report to prepare uniform agarose beads by membrane emulsification, and to investigate the effect of temperature on the size distribution of the droplets and beads. The beads with different size can be prepared by using membranes with different pore size, and the result showed that there was a linear relationship between the average diameter of beads and pore size of the membranes; beads with diameter from 15 to 60 pro were able to obtain in this study. (C) 2007 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Physical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; MONODISPERSED W/O EMULSIONS ; MICROCAPSULES ; MICROSPHERES ; PROTEINS |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000246925700015 |
| 公开日期 | 2013-10-15 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/3535]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biomed Engn, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Qing-Zhu,Wang, Lian-Yan,Ma, Guang-Hui,et al. Preparation of uniform-sized agarose beads by microporous membrane emulsification technique[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE,2007,311(1):118-127. |
| APA | Zhou, Qing-Zhu,Wang, Lian-Yan,Ma, Guang-Hui,&Su, Zhi-Guo.(2007).Preparation of uniform-sized agarose beads by microporous membrane emulsification technique.JOURNAL OF COLLOID AND INTERFACE SCIENCE,311(1),118-127. |
| MLA | Zhou, Qing-Zhu,et al."Preparation of uniform-sized agarose beads by microporous membrane emulsification technique".JOURNAL OF COLLOID AND INTERFACE SCIENCE 311.1(2007):118-127. |
入库方式： OAI收割
来源：过程工程研究所
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