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Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design

文献类型：期刊论文

作者Tian, Miao2,3; Yang, Lingmei2; Lv, Pengmei2; Wang, Zhiyuan2; Fu, Junying2; Miao, Changlin2; Li, Zhibing2; Li, Lianhua2; Liu, Tao1; Du, Wenyi4
刊名BIORESOURCE TECHNOLOGY
出版日期2022-03-01
卷号348页码:9
ISSN号0960-8524
关键词Biodiesel Methanol tolerance N-glycosylation Molecular dynamics Rhizomucor miehei lipase
DOI10.1016/j.biortech.2022.126769
通讯作者Luo, Wen(luowen@ms.giec.ac.cn)
英文摘要Exploiting highly active and methanol-resistant lipase is of great significance for biodiesel production. A semirational directed evolution method combined with N-glycosylation is reported, and all mutants exhibiting higher catalytic activity and methanol tolerance than the wild type (WT). Mutant N267 retained 64% activity after incubation in 50% methanol for 8 h, which was 48% greater than that of WT. The catalytic activity of mutants N267 and N167 was 30- and 71- fold higher than that of WT. Molecular dynamics simulations of N267 showed that the formation of new strong hydrogen bonds between glycan and the protein stabilized the structure of lipase and improved its methanol tolerance. N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil). The engineered ProRML mutant has high potential for commercial biodiesel production.
WOS关键词WASTE COOKING OIL ; N-GLYCOSYLATION ; EXPRESSION
资助项目National Natural Science Foundation of China[51903236] ; National Natural Science Foundation of China[51806225] ; Natural Science Foundation of Guangdong Province of China[2020A1515010513] ; Natural Science Foundation of Guangdong Province of China[2021A1515010325]
WOS研究方向Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
语种英语
出版者ELSEVIER SCI LTD
WOS记录号WOS:000800442200002
资助机构National Natural Science Foundation of China ; Natural Science Foundation of Guangdong Province of China
源URL[http://ir.giec.ac.cn/handle/344007/36680]  
专题中国科学院广州能源研究所
通讯作者Luo, Wen
作者单位1.Guangdong Pharmaceut Univ, Guangdong Prov Key Lab Pharmaceut Bioact Subst, Guangzhou 510006, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Sichuan MoDe Technol Co Ltd, Chengdu 610000, Peoples R China
推荐引用方式
GB/T 7714		 Tian, Miao,Yang, Lingmei,Lv, Pengmei,et al. Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design[J]. BIORESOURCE TECHNOLOGY,2022,348:9.
APA Tian, Miao.,Yang, Lingmei.,Lv, Pengmei.,Wang, Zhiyuan.,Fu, Junying.,...&Luo, Wen.(2022).Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design.BIORESOURCE TECHNOLOGY,348,9.
MLA Tian, Miao,et al."Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design".BIORESOURCE TECHNOLOGY 348(2022):9.

入库方式： OAI收割

来源：广州能源研究所

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