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Potential roles of IFI44 genes in high resistance to Vibrio in hybrids of Argopecten scallops

文献类型：期刊论文

作者Qu, Xiaoxu5; Wang, Xia5; Liu, Bo5; Chen, Min4; Ning, Junhao4; Liu, Haijun3; Liu, Guilong3; Xu, Xin3; Zhang, Xiaotong5; Yu, Kai5
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2023-04-01
卷号135页码:9
ISSN号1050-4648
关键词Argopecten scallops Hybrids Resistance Heterosis IFI44 Vibrio anguillarum
DOI10.1016/j.fsi.2023.108702
通讯作者Lu, Xia(xialu@yic.ac.cn) ; Wang, Chunde(chundewang2007@163.com)
英文摘要Vibrio bacteria are often fatal to aquatic organisms and selection of Vibrio-resistant strains is warranted for aquaculture animals. In this study, we found that hybrids between bay scallops and Peruvian scallops exhibited significantly higher resistance to Vibrio challenge, but little is available on its mechanism. Interferon induced protein 44 (IFI44), a member of the type I interferon (IFN) family, plays an important role in the IFN immune response in invertebrates, which may also participate in the resistance to Vibrio in scallops. To explore the roles of IFI44 genes in the resistance to Vibrio, they were identified and characterized in the bay scallop (designated as AiIFI44), the Peruvian scallop (designated as ApIFI44), and their reciprocal hybrids (designated as AipIFI44 and ApiIFI44, respectively). Their open reading frame (ORF) sequences were all 1434 bp, encoding 477 amino acids, but with large variations among the four genes. The AipIFI44 and ApiIFI44 exhibited higher similarity with ApIFI44 than with AiIFI44. All four genes have a TLDc structural domain with significant variations in sequences among them. Predicted differences in conformation and posttranslational modifications may lead to altered protein activity. We further demonstrated that the AiIFI44, AipIFI44 and ApiIFI44 expressed in all the tested tissues, with the highest expression in the gills and hepatopancreas. In response to Vibrio anguillarum challenge, the profile of mRNA expression of IFI44 gene differed among the bay scallops and the two hybrids. In the bay scallops, it increased at 6 h but dramatically decreased after 12-48 h. However, the mRNA expression of both AipIFI44 and ApiIFI44 decreased at 6 h but continuously increased thereafter and reached the highest value at 48 h. The results in the present study suggest the immune responds of IFI44 in scallops and it may be related to the higher resistance to Vibrio bacterial in hybrids.
WOS关键词EXPRESSION PROFILE ; I INTERFERONS ; KAPPA-B ; PROTEIN ; HEPATOPANCREAS ; HEPATITIS ; IMMUNITY
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000997965900001
资助机构National Natural Science Foundation of China ; Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province ; Scientific and Technological Project of Yantai, Shandong Province ; Earmarked Fund for Shandong Modern Agro-Industry Technology Research System ; Science and Technology Innovation Capacity Improvement Project of Shandong Province ; Natural Science Foundation of Shandong Province
源URL[http://ir.yic.ac.cn/handle/133337/32950]  
专题海岸带生物学与生物资源利用重点实验室
海岸带生物资源高效利用研究与发展中心
通讯作者Lu, Xia; Wang, Chunde
作者单位1.Jiangsu Haitai MariTech Co Ltd, Lianyungang 222144, Peoples R China
2.Jiangsu Baoyuan Biotechnol Co Ltd, Lianyungang 222144, Peoples R China
3.Yantai Spring Sea AquaSeed Co Ltd, Yantai 264006, Peoples R China
4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Shandong, Peoples R China
5.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Qu, Xiaoxu,Wang, Xia,Liu, Bo,et al. Potential roles of IFI44 genes in high resistance to Vibrio in hybrids of Argopecten scallops[J]. FISH & SHELLFISH IMMUNOLOGY,2023,135:9.
APA Qu, Xiaoxu.,Wang, Xia.,Liu, Bo.,Chen, Min.,Ning, Junhao.,...&Wang, Chunde.(2023).Potential roles of IFI44 genes in high resistance to Vibrio in hybrids of Argopecten scallops.FISH & SHELLFISH IMMUNOLOGY,135,9.
MLA Qu, Xiaoxu,et al."Potential roles of IFI44 genes in high resistance to Vibrio in hybrids of Argopecten scallops".FISH & SHELLFISH IMMUNOLOGY 135(2023):9.

入库方式： OAI收割

来源：烟台海岸带研究所

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