Exosomal microRNAs regulate the heat stress response in sea cucumber Apostichopus japonicus
文献类型：期刊论文
| 作者 | Huo, Da3,5,6,7,8; Su, Fang3,4,5,6,7,8; Yang, Hongsheng2,3,4,5,6,7,8 ; Sun, Lina1,3,4,5,6,7,8
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| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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| 出版日期 | 2023 |
| 卷号 | 249页码:10 |
| ISSN号 | 0147-6513 |
| 关键词 | Thermal stress Stress response Defense mechanism Extracellular vesicles MicroRNA Echinoderm |
| DOI | 10.1016/j.ecoenv.2022.114419 |
| 通讯作者 | Sun, Lina(sunlina@qdio.ac.cn) |
| 英文摘要 | Exosomes are small extracellular vesicles that contain nucleic acids such as microRNAs and may participate in important biological processes. We made the initial report of exosomes from sea cucumber Apostichopus japo-nicus, that were classically cup-shaped and had an average size of 74.65 nm, and identified specific exosome biomarkers (HSP70, TSG101, and CD9). We explored changes in the global expression of microRNAs in exosomes from the commercially important A. japonicus under normal conditions and heat-stressed conditions for 3 and 7 d. We found that heat stress increased exosome production and modified the expression profiles of the micro-RNAs that they contained. Novel_mir31, novel_mir132, novel_mir26, miR-92_1, and novel_mir27 were commonly found to be differentially expressed in three comparison groups, indicating their importance in the heat stress response. The microRNA expression levels were validated by qPCR. Function analysis of the target genes of these microRNAs indicated they were involved mainly in replication and repair in the initial response of A. japonicus to heat stress exposure. Conversely, during acclimation to the high temperature conditions, the target genes of the differentially expressed microRNAs were primarily involved in metabolism adjustments. Our results will contribute to a better understanding of the regulatory roles of exosomes in sea cucumber, and provide insights into the functions of sea cucumber exosome-shuttled microRNAs against environmental stresses exacerbated by global warming. |
| 资助项目 | National Natural Science Foundation of China ; Postdoctoral Innova-tive Talents Support Program of Shandong Province ; Major Scientific and Technological Innovation Projects in Shandong Province ; Shandong Provincial Natural Science Foundation ; Special Research Assistant Project of Chinese Academy of Sciences ; Qingdao Postdoctoral Applied Research Project ; Youth Innovation Promotion Association CAS ; [42106106] ; [42030408] ; [SDBX2020006] ; [2019JZZY010812] ; [ZR2021QD013] ; [2020170] ; [2019209] |
| WOS研究方向 | Environmental Sciences & Ecology ; Toxicology |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| WOS记录号 | WOS:000906446700001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/181420]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Sun, Lina |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Innovat Seed Design, Wuhan 430071, Peoples R China 3.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 6.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 7.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 8.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huo, Da,Su, Fang,Yang, Hongsheng,et al. Exosomal microRNAs regulate the heat stress response in sea cucumber Apostichopus japonicus[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2023,249:10. |
| APA | Huo, Da,Su, Fang,Yang, Hongsheng,&Sun, Lina.(2023).Exosomal microRNAs regulate the heat stress response in sea cucumber Apostichopus japonicus.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,249,10. |
| MLA | Huo, Da,et al."Exosomal microRNAs regulate the heat stress response in sea cucumber Apostichopus japonicus".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 249(2023):10. |
入库方式： OAI收割
来源：海洋研究所
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