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Plexin D1 mediates disturbed flow-induced M1 macrophage polarization in atherosclerosis

文献类型：期刊论文

作者Zhang, Suhui4,5; Zhang, Yingqian4; Zhang, Peng3; Wei, Zechen1,2; Ma, Mingrui4,5; Wang, Wei4; Tong, Wei4; Tian, Feng4; Hui, Hui1,2; Tian, Jie2,6,7
刊名HELIYON
出版日期2023-06-01
卷号9期号:6页码:15
关键词Plexin D1 Macrophage polarization Disturbed flow Bifurcation lesions Atherosclerosis
DOI10.1016/j.heliyon.2023.e17314
通讯作者Hui, Hui(hui.hui@ia.ac.cn) ; Tian, Jie(jie.tian@ia.ac.cn) ; Chen, Yundai(cyundai@vip.163.com)
英文摘要Atherosclerosis preferentially develops at bifurcations exposed to disturbed flow. Plexin D1 (PLXND1) responds to mechanical forces and drives macrophage accumulation in atherosclerosis. Here, multiple strategies were used to identify the role of PLXND1 in site-specific atherosclerosis. Using computational fluid dynamics and three-dimensional light-sheet fluorescence-microscopy, the elevated PLXND1 in M1 macrophages was mainly distributed in disturbed flow area of ApoE-/ -carotid bifurcation lesions, and visualization of atherosclerosis in vivo was achieved by targeting PLXND1. Subsequently, to simulate the microenvironment of bifurcation lesions in vitro, we co -cultured oxidized low-density lipoprotein (oxLDL)-treated THP-1-derived macrophages with shear-treated human umbilical vein endothelial cells (HUVECs). We found that oscillatory shear induced the increase of PLXND1 in M1 macrophages, and knocking down PLXND1 inhibited M1 polarization. Semaphorin 3E, the ligand of PLXND1 which was highly expressed in plaques, strongly enhanced M1 macrophage polarization via PLXND1 in vitro. Our findings provide in-sights into pathogenesis in site-specific atherosclerosis that PLXND1 mediates disturbed flow -induced M1 macrophage polarization.
WOS关键词SHEAR-STRESS ; CAROTID BIFURCATION ; PLAQUES ; INFLAMMATION ; ACTIVATION
资助项目National Key Research and Development Program of China[2017YFA0700401] ; National Natural Science Foundation of China[81827808] ; National Natural Science Foundation of China[81730050] ; National Natural Science Foundation of China[81870178] ; National Natural Science Foundation of China[81800221] ; National Natural Science Foundation of China[62027901] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[81671851] ; CAS Youth Innovation Promotion Association[2018167] ; CAS Key Technology Talent Program ; Project of High -Level Talents Team Introduction in Zhuhai City (Zhuhai)[HLHPTP201703]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:001042990400001
出版者CELL PRESS
资助机构National Key Research and Development Program of China ; National Natural Science Foundation of China ; CAS Youth Innovation Promotion Association ; CAS Key Technology Talent Program ; Project of High -Level Talents Team Introduction in Zhuhai City (Zhuhai)
源URL[http://ir.ia.ac.cn/handle/173211/53957]  
专题自动化研究所_中国科学院分子影像重点实验室
通讯作者Hui, Hui; Tian, Jie; Chen, Yundai
作者单位1.Univ Chinese Acad Sci, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing Key Lab Mol Imaging, Beijing 100190, Peoples R China
3.Beijing Jiaotong Univ, Sch Comp & Informat Technol, Beijing 100044, Peoples R China
4.Peoples Liberat Army Gen Hosp, Med Ctr 6, Sr Dept Cardiol, Beijing 100048, Peoples R China
5.Chinese Peoples Liberat Army Gen Hosp, Med Sch Chinese PLA, Beijing 100853, Peoples R China
6.Jinan Univ, Zhuhai Peoples Hosp, Zhuhai Precis Med Ctr, Zhuhai 519000, Peoples R China
7.Beihang Univ, Key Lab Big Data Based Precis Med, Minist Ind & Informat Technol China, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Suhui,Zhang, Yingqian,Zhang, Peng,et al. Plexin D1 mediates disturbed flow-induced M1 macrophage polarization in atherosclerosis[J]. HELIYON,2023,9(6):15.
APA Zhang, Suhui.,Zhang, Yingqian.,Zhang, Peng.,Wei, Zechen.,Ma, Mingrui.,...&Chen, Yundai.(2023).Plexin D1 mediates disturbed flow-induced M1 macrophage polarization in atherosclerosis.HELIYON,9(6),15.
MLA Zhang, Suhui,et al."Plexin D1 mediates disturbed flow-induced M1 macrophage polarization in atherosclerosis".HELIYON 9.6(2023):15.

入库方式： OAI收割

来源：自动化研究所

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