The involvement of calcium in the toxic effect of 4-methylethcathinone on SH-SY5Y cells
文献类型：期刊论文
| 作者 | Zhang, Wei; Cao, Fangqi; Li, Ming; Xu, Zhiwen; Sun, Jing; Huang, Zhiwei; Shi, Ping |
| 刊名 | JOURNAL OF APPLIED TOXICOLOGY
 |
| 出版日期 | 2023 |
| 英文摘要 | Neurotoxicity induced by psychoactive substances is often accompanied by an imbalance of intracellular calcium ions. It is unclear whether calcium ions play a role in the toxicity induced by psychoactive substances. In the present study, we aimed to evaluate the occurrence of calcium dysregulation and its contribution to cytotoxicity in human neurotypic SH-SY5Y cells challenged with a recently developed psychoactive substance 4-methylethcathinone (4-MEC). An increase in the intracellular calcium was detected by inductively coupled plasma atomic emission spectrometry and Fluo-3 AM dye in SH-SY5Y cells after being treated with 4-MEC. The increase of intracellular Ca2+ level mediated G0/G1 cell cycle arrest and ROS/endoplasmic reticulum stress-autophagy signaling pathways to achieve the toxicity of 4-MEC. In particular, N-acetyl-L-cysteine, a classical antioxidant, was found to be a potential treatment for 4-MEC-induced toxicity. Taken together, our results demonstrate that an increase in intracellular calcium content is one of the mechanisms of 4-MEC-induced toxicity. This study provides a molecular basis for the toxicity mechanism and therapeutic intervention of psychoactive substances. The present study aimed to evaluate calcium dysregulation occurrence and its contribution to cytotoxicity in human neurotypic SH-SY5Y cells challenged with a recently developed psychoactive substance 4-MEC. An increase in intracellular calcium was found in SH-SY5Y cells after being treated with 4-MEC. The increase of intracellular Ca2+ level mediated G0/G1 cell cycle arrest and ROS/endoplasmic reticulum stress-autophagy signaling pathways to achieve the toxicity of 4-MEC. N-acetyl-L-cysteine was found to be a potential treatment for 4-MEC-induced toxicity. |
| 源URL | [http://210.75.249.4/handle/363003/61549]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Wei,Cao, Fangqi,Li, Ming,et al. The involvement of calcium in the toxic effect of 4-methylethcathinone on SH-SY5Y cells[J]. JOURNAL OF APPLIED TOXICOLOGY,2023. |
| APA | Zhang, Wei.,Cao, Fangqi.,Li, Ming.,Xu, Zhiwen.,Sun, Jing.,...&Shi, Ping.(2023).The involvement of calcium in the toxic effect of 4-methylethcathinone on SH-SY5Y cells.JOURNAL OF APPLIED TOXICOLOGY. |
| MLA | Zhang, Wei,et al."The involvement of calcium in the toxic effect of 4-methylethcathinone on SH-SY5Y cells".JOURNAL OF APPLIED TOXICOLOGY (2023). |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。