Pancreatic lipase-related protein 2 is selectively expressed by peritubular myoid cells in the murine testis and sustains long-term spermatogenesis
文献类型：期刊论文
| 作者 | Tao, Hai-Ping; Lu, Teng-Fei; Li, Shuang; Jia, Gong-Xue; Zhang, Xiao-Na; Yang, Qi-En; Hou, Yun-Peng |
| 刊名 | CELLULAR AND MOLECULAR LIFE SCIENCES
 |
| 出版日期 | 2023 |
| 卷号 | 80期号:8 |
| 英文摘要 | Spermatogenesis is a complicated process of germ cell differentiation that occurs within the seminiferous tubule in the testis. Peritubular myoid cells (PTMCs) produce major components of the basement membrane that separates and ensures the structural integrity of seminiferous tubules. These cells secrete niche factors to promote spermatogonial stem cell (SSC) maintenance and mediate androgen signals to direct spermatid development. However, the regulatory mechanisms underlying the identity and function of PTMCs have not been fully elucidated. In the present study, we showed that the expression of pancreatic lipase-related protein 2 (Pnliprp2) was restricted in PTMCs in the testis and that its genetic ablation caused age-dependent defects in spermatogenesis. The fertility of Pnliprp2 knockout animals (Pnliprp2(-/-)) was normal at a young age but declined sharply beginning at 9 months. Pnliprp2 deletion impaired the homeostasis of undifferentiated spermatogonia and severely disrupted the development and function of spermatids. Integrated analyses of single-cell RNA-seq and metabolomics data revealed that glyceride metabolism was changed in PTMCs from Pnliprp2(-/-) mice. Further analysis found that 60 metabolites were altered in the sperm of the Pnliprp2(-/-) animals; notably, lipid metabolism was significantly dysregulated. Collectively, these results revealed that Pnliprp2 was exclusively expressed in PTMCs in the testis and played a novel role in supporting continual spermatogenesis in mice. The outcomes of these findings highlight the function of lipid metabolism in reproduction and provide new insights into the regulation of PTMCs in mammals. |
| 源URL | [http://210.75.249.4/handle/363003/61600]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Tao, Hai-Ping,Lu, Teng-Fei,Li, Shuang,et al. Pancreatic lipase-related protein 2 is selectively expressed by peritubular myoid cells in the murine testis and sustains long-term spermatogenesis[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2023,80(8). |
| APA | Tao, Hai-Ping.,Lu, Teng-Fei.,Li, Shuang.,Jia, Gong-Xue.,Zhang, Xiao-Na.,...&Hou, Yun-Peng.(2023).Pancreatic lipase-related protein 2 is selectively expressed by peritubular myoid cells in the murine testis and sustains long-term spermatogenesis.CELLULAR AND MOLECULAR LIFE SCIENCES,80(8). |
| MLA | Tao, Hai-Ping,et al."Pancreatic lipase-related protein 2 is selectively expressed by peritubular myoid cells in the murine testis and sustains long-term spermatogenesis".CELLULAR AND MOLECULAR LIFE SCIENCES 80.8(2023). |
入库方式： OAI收割
来源：西北高原生物研究所
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