Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA
文献类型：期刊论文
| 作者 | Cheng, Jing3; Liu, Tong1; You, Xin4; Zhang, Fa2; Sui, Sen-Fang4; Wan, Xiaohua2; Zhang, Xinzheng3 |
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2023-03-15 |
| 卷号 | 14期号:1页码:9 |
| DOI | 10.1038/s41467-023-36175-y |
| 英文摘要 | Cryo-electron tomography is a major tool used to study the structure of protein complexes in situ. However, the throughput of tilt-series image data collection is still quite low. Here, we show that GisSPA, a GPU accelerated program, can translationally and rotationally localize the target protein complex in cellular lamellae, as prepared with a focused ion beam, using single cryo-electron microscopy images without tilt-series, and reconstruct the protein complex at near-atomic resolution. GisSPA allows high-throughput data collection without the acquisition of tilt-series images and reconstruction of the tomogram, which is essential for high-resolution reconstruction of asymmetric or low-symmetry protein complexes. We demonstrate the power of GisSPA with 3.4-angstrom and 3.9-angstrom resolutions of resolving phycobilisome and tetrameric photosystem II complex structures in cellular lamellae, respectively. In this work, we present GisSPA as a practical tool that facilitates high-resolution in situ protein structure determination. High-resolution in situ protein structure can be solved by cryo-ET, which requires several days of data collection. Here Cheng et al. report GisSPA, a program that may enable determining sub-4 angstrom resolution structures on cellular lamellae within one day of data collection. |
| 资助项目 | National Key R&D Program of China[2021YFA1301501] ; National Key R&D Program of China[2017YFA0504700] ; National Natural Science Foundation of China[31930069] ; National Natural Science Foundation of China[32150010] ; National Natural Science Foundation of China[61932018] ; National Natural Science Foundation of China[32241027] ; National Natural Science Foundation of China[62072441] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB37040101] ; Key Research Program of Frontier Sciences at the Chinese Academy of Sciences[ZDBS-LY-SM003] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | NATURE PORTFOLIO |
| WOS记录号 | WOS:001001760400005 |
| 源URL | [http://119.78.100.204/handle/2XEOYT63/21189]  |
| 专题 | 中国科学院计算技术研究所期刊论文_英文 |
| 通讯作者 | Wan, Xiaohua; Zhang, Xinzheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Comp Technol, High Performance Comp Res Ctr, Beijing 100190, Peoples R China 2.Beijing Inst Technol, Beijing 100081, Peoples R China 3.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromolecules, Natl Lab Biomacromolecules, Beijing 100101, Peoples R China 4.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Sch Life Sci, State Key Lab Membrane Biol, Beijing 100084, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, Jing,Liu, Tong,You, Xin,et al. Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA[J]. NATURE COMMUNICATIONS,2023,14(1):9. |
| APA | Cheng, Jing.,Liu, Tong.,You, Xin.,Zhang, Fa.,Sui, Sen-Fang.,...&Zhang, Xinzheng.(2023).Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA.NATURE COMMUNICATIONS,14(1),9. |
| MLA | Cheng, Jing,et al."Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA".NATURE COMMUNICATIONS 14.1(2023):9. |
入库方式： OAI收割
来源：计算技术研究所
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