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Au-Fe3O4 nanozyme coupled with CRISPR-Cas12a for sensitive and visual antibiotic resistance diagnosing

文献类型：期刊论文

作者Chen, Haoxiang2; Li, Bangying3; Shi, Shangyi4,5; Zhou, Tao2; Wang, Xiumin3; Wang, Zuyong6; Zhou, Xi2; Wang, Miao2; Shi, Wei2; Ren, Lei1,2
刊名ANALYTICA CHIMICA ACTA
出版日期2023-04-22
卷号1251页码:10
ISSN号0003-2670
关键词CRISPR Cas12a Colorimetric assay Antibiotic resistance bacteria Antibiotic resistance gene Nanozyme Pathogen detection
DOI10.1016/j.aca.2023.341014
英文摘要The accumulation and spread of antibiotic resistance bacteria (ARB) in the environment may accelerate the formation of superbugs and seriously threaten the health of all living beings. The timeliness and accurate diagnosing of antibiotic resistance is essential to controlling the propagation of superbugs in the environment and formulating effective public health management programs. Herein, we developed a speedy, sensitive, ac-curate, and user-friendly colorimetric assay for antibiotic resistance, via a synergistic combination of the peroxidase-like property of the Au-Fe3O4 nanozyme and the specific gene identification capability of the CRISPR-Cas12a. Once the CRISPR-Cas12a system recognizes a target resistance gene, it activates its trans -cleavage activity and subsequently releases the Au-Fe3O4 nanozymes, which oxidizes the 3,3,5,5-tetramethyl-benzidine (TMB) with color change from transparent to blue. The diagnosing signals could be captured and analyzed by a smartphone. This method detected kanamycin-resistance genes, ampicillin-resistance genes, and chloramphenicol-resistance genes by simple operation steps with high sensitivity (<0.1 CFU mu L-1) and speedi-ness (<1 h). This approach may prove easy for the accurate and sensitive diagnosis of the ARGs or ARB in the field, thus surveilling and controlling the microbial water quality flexibly and efficiently.
资助项目National Natural Science Foundation of China[31870994] ; National Natural Science Foundation of China[U1904206] ; National Natural Science Foundation of China[22172132] ; Fujian Provincial Science and Technology Cooperation Project[20210002] ; Natural Science Foundation of Xiamen, China[3502Z20227010]
WOS研究方向Chemistry
语种英语
出版者ELSEVIER
WOS记录号WOS:000990752700001
源URL[http://119.78.100.204/handle/2XEOYT63/21419]  
专题中国科学院计算技术研究所期刊论文_英文
通讯作者Shi, Wei; Ren, Lei
作者单位1.Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China
2.Xiamen Univ, Coll Mat, Res Ctr Biomed Engn Xiamen, Dept Biomat,Higher Educ Key Lab Biomed Engn Fujian, Xiamen 361005, Peoples R China
3.Xiamen Univ, Sch Pharmaceut Sci, Xiamen 361102, Peoples R China
4.Chinese Acad Sci, Inst Comp Technol, State Key Lab Comp Architecture, Beijing 100190, Peoples R China
5.Univ Chinese Acad Sci, Sch Comp Sci & Technol, Beijing 100049, Peoples R China
6.Hunan Univ, Coll Mat Sci & Engn, Changsha 410072, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Haoxiang,Li, Bangying,Shi, Shangyi,et al. Au-Fe3O4 nanozyme coupled with CRISPR-Cas12a for sensitive and visual antibiotic resistance diagnosing[J]. ANALYTICA CHIMICA ACTA,2023,1251:10.
APA Chen, Haoxiang.,Li, Bangying.,Shi, Shangyi.,Zhou, Tao.,Wang, Xiumin.,...&Ren, Lei.(2023).Au-Fe3O4 nanozyme coupled with CRISPR-Cas12a for sensitive and visual antibiotic resistance diagnosing.ANALYTICA CHIMICA ACTA,1251,10.
MLA Chen, Haoxiang,et al."Au-Fe3O4 nanozyme coupled with CRISPR-Cas12a for sensitive and visual antibiotic resistance diagnosing".ANALYTICA CHIMICA ACTA 1251(2023):10.

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来源：计算技术研究所

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