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Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum

文献类型：期刊论文

作者Yanga, Wenting1,2,4; Zhou, Lu1,2,3; Zhuo, Jintao1,2,3; Li, Xin1,2,3; Wu, Songcui1,2; Yu, Guihong; Gao, Shan1,2; Wang, Guangce1,2
刊名ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
出版日期2023-09-01
卷号75页码:7
ISSN号2211-9264
关键词Phaeodactylum tricornutum Secretory antimicrobial peptide Antibacterial
DOI10.1016/j.algal.2023.103270
通讯作者Gao, Shan(shangao@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要Antimicrobial peptides (AMPs) are small-molecule polypeptides that occur widely in nature, have a broad antibacterial spectrum, and do not induce drug resistance in bacteria. Therefore, they are potential substitutes for antibiotics. However, there have been few reports of the production of the AMPs secreted by diatoms. Phaeodactylum tricornutum is a model diatom species for which protocols for its transformation are well established, and it can be directly used as fish and shrimp bait. In this study, we used P. tricornutum as a cell factory to produce secreted AMPs extracellularly. We also constructed an expression vector containing the secretory promoter and signal peptide of the Scygonadin gene, and successfully expressed AMPs in P. tricornutum, confirmed at the levels of DNA, RNA, and protein. Bacteriostatic experiments showed that the secreted AMPs exerted an obvious antibacterial effect on Kocuria sp. and Trelief5 alpha Chemical Competent Cells. This study demonstrates that P. tricornutum effectively expresses and secretes AMPs with obvious antibacterial effects.
WOS关键词TRANSFORMATION ; MECHANISMS ; GENE
资助项目National Natural Science Founda- tion of China[41976097] ; National Key R & D Program of China[2018YFD0901500]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
出版者ELSEVIER
WOS记录号WOS:001083333200001
源URL[http://ir.qdio.ac.cn/handle/337002/181622]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Gao, Shan; Wang, Guangce
作者单位1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
4.Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Yanga, Wenting,Zhou, Lu,Zhuo, Jintao,et al. Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2023,75:7.
APA Yanga, Wenting.,Zhou, Lu.,Zhuo, Jintao.,Li, Xin.,Wu, Songcui.,...&Wang, Guangce.(2023).Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,75,7.
MLA Yanga, Wenting,et al."Expression of secreted antimicrobial peptide in a marine diatom-Phaeodactylum tricornutum".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 75(2023):7.

入库方式： OAI收割

来源：海洋研究所

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