Zebrafish (Danio rerio) TR beta- and TTR-based electrochemical biosensors: Construction and application for the evaluation of thyroid-disrupting activity of bisphenols
文献类型：期刊论文
| 作者 | Li, Yuejiao1; Zhang, Zhenzhong1; Wang, Jun1; Shan, Yeqi2,3,4; Tian, Hua1; Cui, Pengfei1; Ru, Shaoguo1 |
| 刊名 | ENVIRONMENTAL POLLUTION
 |
| 出版日期 | 2023-08-01 |
| 卷号 | 330页码:9 |
| 关键词 | Thyroid-disrupting chemicals Thyroid hormone receptor beta Transthyretin In vitro screening Assay Electrochemical biosensor |
| ISSN号 | 0269-7491 |
| DOI | 10.1016/j.envpol.2023.121745 |
| 通讯作者 | Ru, Shaoguo(rusg@ouc.edu.cn) |
| 英文摘要 | Thyroid-disrupting chemicals (TDCs) have received increasing concerns because of their negative health impacts on both wildlife and humans. This study aimed to develop in vitro screening assays for TDCs based on thyroid hormone receptor beta (TR beta) and transthyretin (TTR) proteins. Firstly, the recombinant ligand-binding domain of TR beta (TR beta-LBD) and TTR proteins of zebrafish were produced by eukaryotic expression system and then used as bio-recognition components to construct electrochemical biosensors. In the biosensors, the supported bilayer lipid membrane (s-BLM) was used as a matrix to immobilize proteins, and gold nanoflowers (AuNFs) were used to improve the sensitivity by increasing electroactive surface area. Under the optimizing conditions, the zfTR beta-LBD/AuNFs/s-BLM/GCE biosensor had a detection range of 0.23 nM-1.92 mu M and a detection limit of 0.07 nM for triiodothyronine (T-3), while the zfTTR/AuNFs/s-BLM/GCE biosensor had a detection range of 0.46 nM-3.84 mu M, with a detection limit of 0.13 nM. Based on the constructed biosensors, the order of T-3 equivalent concentrations of bisphenols was BPA approximate to BPS > BPF > BPAF approximate to BPAP > BPZ, which was similar to the results of recombinant TR beta two-hybrid yeast assay. Furthermore, the reliability of the biosensors was validated by molecular docking, in which BPA and BPS showed higher binding affinity to zfTR beta-LBD. Therefore, this study provided a valuable tool for efficiently screening TDCs. |
| WOS关键词 | ESTROGENIC ACTIVITY ; IN-VITRO ; IDENTIFICATION ; CHEMICALS ; DRINKING ; PROTOCOL ; PROTEIN |
| 资助项目 | National Natural Science Foundation of China[41976139] ; National Natural Science Foundation of China[21607144] |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:000998593300001 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/182255]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Ru, Shaoguo |
| 作者单位 | 1.Ocean Univ China, Coll Marine Life Sci, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 3.Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Chinese Acad Sci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yuejiao,Zhang, Zhenzhong,Wang, Jun,et al. Zebrafish (Danio rerio) TR beta- and TTR-based electrochemical biosensors: Construction and application for the evaluation of thyroid-disrupting activity of bisphenols[J]. ENVIRONMENTAL POLLUTION,2023,330:9. |
| APA | Li, Yuejiao.,Zhang, Zhenzhong.,Wang, Jun.,Shan, Yeqi.,Tian, Hua.,...&Ru, Shaoguo.(2023).Zebrafish (Danio rerio) TR beta- and TTR-based electrochemical biosensors: Construction and application for the evaluation of thyroid-disrupting activity of bisphenols.ENVIRONMENTAL POLLUTION,330,9. |
| MLA | Li, Yuejiao,et al."Zebrafish (Danio rerio) TR beta- and TTR-based electrochemical biosensors: Construction and application for the evaluation of thyroid-disrupting activity of bisphenols".ENVIRONMENTAL POLLUTION 330(2023):9. |
入库方式： OAI收割
来源：海洋研究所
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