Effect of ovarian fluid on sperm performance in teleost with internal and external fertilization strategies
文献类型：期刊论文
| 作者 | Wang, Xueying1,2 ; Liu, Qinghua1,2; Zhou, Li1,2,3; Song, Zongcheng4; Li, Jun1,2
|
| 刊名 | THERIOGENOLOGY
 |
| 出版日期 | 2023-08-01 |
| 卷号 | 206页码:189-196 |
| 关键词 | Turbot Black rockfish Sperm activation medium |
| ISSN号 | 0093-691X |
| DOI | 10.1016/j.theriogenology.2023.05.003 |
| 通讯作者 | Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn) |
| 英文摘要 | Ovarian fluid is essential for successful fertilization by maintaining the viability, motility, and velocity of sperm. The organic compounds and inorganic ions in ovarian fluid significantly influence spermatozoa's motility, velocity, and longevity. However, the effect of ovarian fluid on sperm performance is limited in teleost fish. In this study, the effect of ovarian fluid on sperm performance and its components in external fertilization species (Scophthalmus maximus, turbot) and internal fertilization species (Sebastes schlegelii, black rockfish) was investigated using computer-assisted sperm analysis, high-performance liquid chromatography, and metabolome analysis. The ovarian fluid had a distinct and species-specific effect on both species. In the black rockfish, the ovarian fluid from turbot significantly increased sperm motility (74.07% +/- 4.09%), as well as VCL (45 +/- 1.67 mm/s), VAP (40.17 +/- 1.6 mm/s), and VSL (36.67 +/- 1.86 mm/s), and longevity (352 +/- 11.31 min) (P < 0.05). In the turbot, only the longevity (71.33 +/- 5.69 min) and fertilization rate (65.27% +/- 11.59%) showed significantly improvement (P < 0.05). The ovarian fluid was rich in organic compounds, suggesting enrichment in the glycolysis/gluconeogenesis pathways. The results suggest that glycometabolism plays a crucial role in improving sperm performance in teleost with internal fertilization. Thus, incorporating ovarian fluid into the sperm activation medium can enhance artificial fertilization in fish breeding.(c) 2023 Elsevier Inc. All rights reserved. |
| WOS关键词 | TURBOT SCOPHTHALMUS-MAXIMUS ; ONCORHYNCHUS-MYKISS WALBAUM ; RAINBOW-TROUT ; BIOCHEMICAL-COMPOSITION ; MOTILITY ; ACTIVATION ; GLYCOLYSIS ; QUALITY |
| 资助项目 | National Natural Science Foundation of China[31802278] ; National Key R &D Program of China[2018YFD0901204] ; National Key R &D Program of China[2018YFD0901205] ; China Agriculture Research System[CARS-47] ; Major science and technology for scientific and technological innovation projects (Shandong)[2019JZZY020710] |
| WOS研究方向 | Reproductive Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:001008080600001 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/182264]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Qinghua; Li, Jun |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xueying,Liu, Qinghua,Zhou, Li,et al. Effect of ovarian fluid on sperm performance in teleost with internal and external fertilization strategies[J]. THERIOGENOLOGY,2023,206:189-196. |
| APA | Wang, Xueying,Liu, Qinghua,Zhou, Li,Song, Zongcheng,&Li, Jun.(2023).Effect of ovarian fluid on sperm performance in teleost with internal and external fertilization strategies.THERIOGENOLOGY,206,189-196. |
| MLA | Wang, Xueying,et al."Effect of ovarian fluid on sperm performance in teleost with internal and external fertilization strategies".THERIOGENOLOGY 206(2023):189-196. |
入库方式： OAI收割
来源：海洋研究所
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