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Identification and characterization of IgNAR and VNAR repertoire from the ocellate spot skate (Okamejei kenojei)

文献类型：期刊论文

作者Wen, Jianqing1,2; Gong, Jinyu2,3; Li, Pengwei1; Deng, Penghui1,2; Sun, Mengsi4; Wu, Yujie4; Tian, Chenxi2,3; Wang, Hao1; Bi, Yunchen1,2,5
刊名FRONTIERS IN MARINE SCIENCE
出版日期2023-05-24
卷号10页码:13
关键词IgNAR VNAR skate VNAR structure antibody diversity
DOI10.3389/fmars.2023.1183744
通讯作者Wang, Hao(wanghao@qdio.ac.cn) ; Bi, Yunchen(yunchenbi@qdio.ac.cn)
英文摘要Elasmobranchs are crucial for comparative studies of evolution, as they belong to the most ancient vertebrate lineages that survived numerous extinction events and persist until today. The immunoglobulin new antigen receptor (IgNAR) found in sharks and heavy-chain-only antibody (HCAb) found in camelidae are products of convergent evolution. Although it was previously believed that IgNAR emerged 220 million years ago, before the divergence of sharks and skates, there is limited evidence to support this. In this study, we provide data supporting the existence of IgNAR in the ocellate spot skate (Okamejei kenojei) mononuclear cell transcriptome and peripheral blood serum. Additionally, we characterize the germline gene configuration of the ocellate spot skate IgNAR V domain. The ocellate spot skate IgNAR structure prediction and VNAR crystal structure exhibit high similarity to their shark counterparts. These data strongly suggest that IgNAR in both sharks and skates share a common ancestor. Sequencing of the ocellate spot skate VNAR repertoire provided crucial data for further understanding of the IgNAR generation. Notably, we discovered that approximately 99% of the ocellate spot skate VNARs belonged to type IV. This represents an exceptionally high proportion of type IV within the VNAR repertoire, which has not been documented in previously studied elasmobranchs. This unique characteristic of the ocellate spot skate VNAR adds essential structural diversity to the naive VNAR library from elasmobranchs and could potentially benefit the development of pharmaceutical drugs.
WOS关键词MULTIPLE SEQUENCE ALIGNMENT ; VARIABLE DOMAIN ANTIBODIES ; ANTIGEN RECEPTOR IGNAR ; LIGHT-CHAIN GENES ; SHARK IMMUNOGLOBULIN ; STRUCTURAL-ANALYSIS ; PROTEIN ; REARRANGEMENT ; DIVERSITY ; EXCLUSION
资助项目National Natural Science Foundation of China[41876168] ; Taishan Young Scholar Program of Shandong Province[tsqn201812108] ; Hefei Science Center, CAS[2020HSC-UE018]
WOS研究方向Environmental Sciences & Ecology ; Marine & Freshwater Biology
语种英语
出版者FRONTIERS MEDIA SA
WOS记录号WOS:001005205000001
源URL[http://ir.qdio.ac.cn/handle/337002/182276]  
专题海洋研究所_实验海洋生物学重点实验室
海洋研究所_海洋生态与环境科学重点实验室
通讯作者Wang, Hao; Bi, Yunchen
作者单位1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Coll Marine Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Beijing Inst Genom, Key Lab Genom & Precis Med, Beijing, Peoples R China
4.Shenzhen Bay Lab, Dept Core Facil, Biochem Core, Shenzhen, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Wen, Jianqing,Gong, Jinyu,Li, Pengwei,et al. Identification and characterization of IgNAR and VNAR repertoire from the ocellate spot skate (Okamejei kenojei)[J]. FRONTIERS IN MARINE SCIENCE,2023,10:13.
APA Wen, Jianqing.,Gong, Jinyu.,Li, Pengwei.,Deng, Penghui.,Sun, Mengsi.,...&Bi, Yunchen.(2023).Identification and characterization of IgNAR and VNAR repertoire from the ocellate spot skate (Okamejei kenojei).FRONTIERS IN MARINE SCIENCE,10,13.
MLA Wen, Jianqing,et al."Identification and characterization of IgNAR and VNAR repertoire from the ocellate spot skate (Okamejei kenojei)".FRONTIERS IN MARINE SCIENCE 10(2023):13.

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来源：海洋研究所

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