中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 海洋研究所
  3. 中国科学院海洋研究所
  4. 实验海洋生物学重点实验室
Appropriate carbon-nitrogen ratio is beneficial to the accumulation of 9-cis-beta-carotene during Dunaliella salina cultivation

文献类型：期刊论文

作者Jia, Shuao1,2,3,4; Wu, Songcui1,2,3; Liu, Xuehua1,2,3; Gu, Wenhui1,2,3; Wang, Guangce1,2,3
刊名JOURNAL OF APPLIED PHYCOLOGY
出版日期2023-06-10
页码16
ISSN号0921-8971
关键词Dunaliella salina Carbon-nitrogen ratio (C/N) 9-cis beta-carotene Carotenoids biosynthesis Metabolic regulation
DOI10.1007/s10811-023-03008-w
通讯作者Gu, Wenhui(wenhuigu@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要The halotolerant green alga Dunaliella salina is widely cultivated in the industrial-scale production of natural beta-carotene (containing 9-cis and all-trans isomers) due to its high carotenoid content. The management of environmental factors such as nitrogen deficiency is an efficient way to induce the production of beta-carotene. However, it is not clear how changes in nitrogen concentration and the resulting changes in the carbon-nitrogen (C/N) ratio affect the beta-carotene isomer configuration ratio and content. In this study, D. salina HG01 was cultured under different C/N ratios, growth parameters, beta-carotene content and the ratio of two isomers, intracellular substance content, and antioxidant enzyme activity were determined. The results showed that although a large amount of beta-carotene was accumulated in algae cells under a high C/N ratio, the high C/N ratio was not conducive to the increase of beta-carotene content in unit culture volume and the proportion of 9-cis-beta-carotene. However, the beta-carotene content in per unit of culture volume (14.70 mu g mL(-1)) under a medium C/N ratio and the proportion of 9-cis-beta-carotene (39.31%) under a low C/N ratio were relatively higher. In addition, transcriptome sequencing revealed changes in the expression of genes related to photosynthesis, carbon and nitrogen. This study integrates the physiological and transcriptomics data related with beta-carotene configuration changes in D. salina under different C/N ratios, which has practical implications for the industrial production of beta-carotene. By optimizing the C/N ratio during cultivation, it is possible to enhance beta-carotene content and improve the desired 9-cis isomer proportion. These findings can contribute to the development of more efficient and cost-effective production methods for natural beta-carotene, thereby benefiting D. salinabased beta-carotene industry.
WOS关键词BETA-CAROTENE ; GENETIC-TRANSFORMATION ; TRANSCRIPTOME ANALYSIS ; ANTIOXIDANT ACTIVITY ; ALL-TRANS ; ALGA ; BARDAWIL ; BIOTECHNOLOGY ; DWARF27 ; MODE
资助项目Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; National Natural Science Foundation of China[42206097] ; National Natural Science Foundation of China[42276146] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science ; Marine Bio-logical Carbon Cycling Research ; Chinese Academy of Sciences[COMS2019Q02]
WOS研究方向Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种英语
出版者SPRINGER
WOS记录号WOS:001006605400001
源URL[http://ir.qdio.ac.cn/handle/337002/182311]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Gu, Wenhui; Wang, Guangce
作者单位1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Chinese Acad Sci, Qingdao, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Jia, Shuao,Wu, Songcui,Liu, Xuehua,et al. Appropriate carbon-nitrogen ratio is beneficial to the accumulation of 9-cis-beta-carotene during Dunaliella salina cultivation[J]. JOURNAL OF APPLIED PHYCOLOGY,2023:16.
APA Jia, Shuao,Wu, Songcui,Liu, Xuehua,Gu, Wenhui,&Wang, Guangce.(2023).Appropriate carbon-nitrogen ratio is beneficial to the accumulation of 9-cis-beta-carotene during Dunaliella salina cultivation.JOURNAL OF APPLIED PHYCOLOGY,16.
MLA Jia, Shuao,et al."Appropriate carbon-nitrogen ratio is beneficial to the accumulation of 9-cis-beta-carotene during Dunaliella salina cultivation".JOURNAL OF APPLIED PHYCOLOGY (2023):16.

入库方式： OAI收割

来源：海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。