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Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments

文献类型：期刊论文

作者Zhou, Cong1,2,3,4,5,6; Yang, Mei-jie1,2,3,4,5,6; Hu, Zhi1,2,3,4,5,6; Zou, Yan7; Shi, Pu1,2,3,4,5,6; Li, Yong-ren8; Guo, Yong-jun8; Song, Hao1,2,3,4,6,9; Zhang, Tao1,2,3,4,6,9
刊名MARINE ENVIRONMENTAL RESEARCH
出版日期2023-11-01
卷号192页码:11
ISSN号0141-1136
关键词Hyposalinity Hypersalinity Transcriptome Autophagy Free amino acids
DOI10.1016/j.marenvres.2023.106198
通讯作者Song, Hao(haosong@qdio.ac.cn) ; Zhang, Tao(tzhang@qdio.ac.cn)
英文摘要Marine bivalves in intertidal zones and land-based seawater ponds are constantly subjected to a wide range of salinity fluctuations due to heavy rainfall, intense drought, and human activities. As osmoconformers, bivalves rely primarily on rapid release or accumulation of free amino acids (FAAs) for osmoregulation. Euryhaline bivalves are capable of withstanding hyposaline and hypersaline environments through regulation of physiology, metabolism, and gene expression. However, current understanding of the molecular mechanisms underlying osmoregulation and salinity adaptation in euryhaline bivalves remains largely limited. In this study, RNA-seq, WGCNA and flow cytometric analysis were performed to investigate the physiological responses of hard clams (Mercenaria mercenaria) to acute short-term hyposalinity (AL) and hypersalinity (AH), and chronic long-term hyposalinity (CL) and hypersalinity (CH) stress. We found that amino acids biosynthesis was significantly inhibited and aminoacyl-tRNA biosynthesis was augmented to decrease intracellular osmolarity during hypo saline exposure. Under CH, numerous autophagy-related genes (ATGs) were highly expressed, and the autophagy activity of gill cells were significantly up-regulated. A significant decrease in total FAAs content was observed in gills after NH4Cl treatment, indicating that autophagy was crucial for osmoregulation in hard clams during prolonged exposure to hypersaline environments. To prevent premature or unnecessary apoptosis, the expression of cathepsin L was inhibited under AL and AH, and inhibitors of apoptosis was augmented under CL and CH. Additionally, neuroendocrine regulation was involved in salinity adaption in hard clams. This study provides novel insights into the physiological responses of euryhaline marine bivalves to hyposaline and hypersaline environments.
WOS关键词OYSTER CRASSOSTREA-GIGAS ; PACIFIC OYSTER ; MYTILUS-GALLOPROVINCIALIS ; ENDOPLASMIC-RETICULUM ; MACOMA-BALTHICA ; STRESS ; EXPRESSION ; SALINITY ; ADAPTATION ; MECHANISMS
资助项目Tianjin science and technology commission project[20YFZCSN00240] ; Tianjin Agricultural Committee Project[202103010] ; Key Research and Development Program of Guangxi Province[LMEES-CTSP-2018-1] ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao Pilot National Laboratory for Marine Science and Technology[2021QNRC001] ; Young Elite Scientists Sponsorship Program by CAST[CARS-49] ; Youth Innovation Promotion Association CAS ; earmarked fund for CARS ; [2021AB34014]
WOS研究方向Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Toxicology
语种英语
出版者ELSEVIER SCI LTD
WOS记录号WOS:001085440300001
源URL[http://ir.qdio.ac.cn/handle/337002/181619]  
专题海洋研究所_海洋生态与环境科学重点实验室
通讯作者Song, Hao; Zhang, Tao
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
7.Marine Sci Res Inst Shandong Prov, Qingdao 266100, Peoples R China
8.Tianjin Agr Univ, Fisheries Coll, Tianjin Key Lab Aquaecol & Aquaculture, Tianjin 300384, Peoples R China
9.7 Nanhai Rd, Qingdao, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Cong,Yang, Mei-jie,Hu, Zhi,et al. Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments[J]. MARINE ENVIRONMENTAL RESEARCH,2023,192:11.
APA Zhou, Cong.,Yang, Mei-jie.,Hu, Zhi.,Zou, Yan.,Shi, Pu.,...&Zhang, Tao.(2023).Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments.MARINE ENVIRONMENTAL RESEARCH,192,11.
MLA Zhou, Cong,et al."Autophagy contributes to increase the content of intracellular free amino acids in hard clam (Mercenaria mercenaria) during prolonged exposure to hypersaline environments".MARINE ENVIRONMENTAL RESEARCH 192(2023):11.

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来源：海洋研究所

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