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Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection

文献类型：期刊论文

作者Zeng, Yan1,2,3; Qi, Peng1,2,3; Chen, Jiawei1,2,3; Wang, Peng1,2,3; Zhang, Dun1,2,3
刊名SENSORS AND ACTUATORS B-CHEMICAL
出版日期2023-12-01
卷号396页码:7
关键词DNA hyperbranched nanostructure SERS Target aided Bacterial 16 S rDNA genes
DOI10.1016/j.snb.2023.134423
通讯作者Qi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn)
英文摘要Quantity analysis of bacterial 16 S rDNA gene is still a challenge. Here we successfully achieved bacterial 16 S rDNA gene SERS analysis with target aided self-assembly of DNA hyperbranched nanostructures. Catalytic hairpin assembly (CHA) was first introduced for self-assembly of branched nanostructures, including target bacterial DNA served as trigger probe and three hairpin probes for further target activated cross opening. Rolling cycle amplification (RCA) was then introduced by using a circular DNA as template and single stranded branches of the formed branched nanostructures as primer, forming a concatemer containing multiple copies of tandem repeating segments with same sequences complementary to the circular templates. After that, thousands of Raman-Au nanoprobe would hybridize this long single stranded DNAs for DNA-AuNPs nanoaggretates assembly. By using SERS signal collection, target bacterial DNAs would be successfully analyzed with ultra-sensitivity as low as 15.0 fM. Furthermore, selectivity test for targets with mismatched DNA sequences and other bacterial 16 S rDNA genes show good results. This sensing method was extended to quantify target genes in human plasma samples, showing its excellent applicability in complex samples.
WOS关键词GOLD NANOPARTICLES ; AMPLIFICATION
资助项目National Natural Science Foundation of China[42376208] ; National Natural Science Foundation of China[41922040]
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种英语
WOS记录号WOS:001075737400001
出版者ELSEVIER SCIENCE SA
源URL[http://ir.qdio.ac.cn/handle/337002/181706]  
专题海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者Qi, Peng; Zhang, Dun
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Open studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Zeng, Yan,Qi, Peng,Chen, Jiawei,et al. Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection[J]. SENSORS AND ACTUATORS B-CHEMICAL,2023,396:7.
APA Zeng, Yan,Qi, Peng,Chen, Jiawei,Wang, Peng,&Zhang, Dun.(2023).Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection.SENSORS AND ACTUATORS B-CHEMICAL,396,7.
MLA Zeng, Yan,et al."Target aided self-assembly of DNA hyperbranched nanostructures for bacterial 16 S ribosomal DNA gene SERS detection".SENSORS AND ACTUATORS B-CHEMICAL 396(2023):7.

入库方式： OAI收割

来源：海洋研究所

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