中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 海洋研究所
  3. 中国科学院海洋研究所
  4. 海洋腐蚀与防护研究发展中心
Colorimetric analysis of sulfate-reducing bacterial DNA based on catalytic hemin/G-quadruplex loaded rigid DNA triangle

文献类型：期刊论文

作者Zhou, Yanan1,2,3,4; Zeng, Yan1,2,3; Wang, Peng1,2; Qi, Peng1,2,3; Sun, Yan1,2,3; Zhang, Dun1,2,3
刊名SENSORS AND ACTUATORS B-CHEMICAL
出版日期2023-09-01
卷号390页码:8
关键词Rigid DNA triangle Hemin G-quadruplex Sulfate -reducing bacteria
DOI10.1016/j.snb.2023.133854
通讯作者Zeng, Yan(zengyan@qdio.ac.cn) ; Zhang, Dun(zengyan@qdio.ac.cn)
英文摘要Here a colorimetric sensing platform for sulfate-reducing bacterial DNA analysis was described by using a catalytic hemin/G-quadruplex loaded rigid DNA triangle assembly. This two-dimensional rigid DNA triangle structure was assembled by target bacterial DNA and three hairpin beacon probes. As three tips of hairpin probes carry specific recognition sites for G-quadruplex formation, target bacterial DNA trigger the opening of hairpin probes, not only forming a steady rigid DNA triangle but also activating the hemin/G-quadruplex horseradish peroxidase mimicking DNAzyme. The resultant hemin/G-quadruplex loaded rigid DNA triangle assembly catalyzes a colorimetric reaction with the signal intensity correlated to target concentrations, achieving target bacterial DNA detection with a detection limit as low as 23.99 fM. We further analyzed actual bacteria with this system, by acquiring bacterial DNA including bacteria-specific sequences with polymerase chain reaction (PCR), with the detection limit down to 1 cfu/mL. The system shows good selectivity for the target and resistance to interference, highly stable performance for serum samples application, showing great potential for application in the fields of genetic analysis, environmental monitoring, and medical testing.
WOS关键词LABEL-FREE
资助项目National Natural Science Foundation of China[41906037] ; National Natural Science Foundation of China[41876101]
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种英语
WOS记录号WOS:001010022200001
出版者ELSEVIER SCIENCE SA
源URL[http://ir.qdio.ac.cn/handle/337002/182308]  
专题海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者Zeng, Yan; Zhang, Dun
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Yanan,Zeng, Yan,Wang, Peng,et al. Colorimetric analysis of sulfate-reducing bacterial DNA based on catalytic hemin/G-quadruplex loaded rigid DNA triangle[J]. SENSORS AND ACTUATORS B-CHEMICAL,2023,390:8.
APA Zhou, Yanan,Zeng, Yan,Wang, Peng,Qi, Peng,Sun, Yan,&Zhang, Dun.(2023).Colorimetric analysis of sulfate-reducing bacterial DNA based on catalytic hemin/G-quadruplex loaded rigid DNA triangle.SENSORS AND ACTUATORS B-CHEMICAL,390,8.
MLA Zhou, Yanan,et al."Colorimetric analysis of sulfate-reducing bacterial DNA based on catalytic hemin/G-quadruplex loaded rigid DNA triangle".SENSORS AND ACTUATORS B-CHEMICAL 390(2023):8.

入库方式： OAI收割

来源：海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。