Multiple heart-cutting two-dimensional liquid chromatography/charged aerosol detector assay of ginsenosides for quality evaluation of ginseng from diverse Chinese patent medicines
文献类型：期刊论文
| 作者 | Xu, Xiao-yan3,4; Jiang, Mei-ting3,4; Wang, Yu3,4; Sun, He3,4; Jing, Qi3,4; Li, Xiao-hang3,4; Xu, Bei3,4; Zou, Ya-dan3,4; Yu, He-shui2,3,4; Li, Zheng2,3,4 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2023-10-11 |
| 卷号 | 1708页码:10 |
| 关键词 | Multiple heart -cutting two-dimensional liquid chromatography Charged aerosol detector Quantitative assay Ginseng Chinese patent medicine |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2023.464344 |
| 通讯作者 | Guo, De-an(daguo@simm.ac.cn) ; Yang, Wen-zhi(wzyang0504@tjutcm.edu.cn) |
| 英文摘要 | For quality control of Chinese patent medicines (CPMs) containing the same herbal medicine or different herbal medicines that have similar chemical composition, current '' one standard for one species '' research mode leads to poor universality of the analytical approaches unfavorable to discriminate easily confused species. Herein, we were aimed to elaborate a multiple heart-cutting two-dimensional liquid chromatography/charged aerosol detector (MHC-2DLC/CAD) approach to quantitatively assess ginseng from multiple CPMs. Targeting baseline resolution of 16 ginsenosides (noto-R1/Rg1/Re/Rf/Ra2/Rb1/Rc/Ro/Rb2/Rb3/Rd/Rh1/Rg2/Rg3/Rg3(R)/24 (R)-p-F11), experiments were conducted to optimize key parameters and validate its performance. A Poroshell 120 EC-C18 column and an XBridge Shield RP18 column were separately utilized in the first-dimensional (D-1) and the second-dimensional (D-2) chromatography. Eight consecutive cuttings could achieve good separation of 16 ginsenosides within 85 min. The developed MHC-2DLC/CAD method showed good linearity (R-2 > 0.999), repeatability (RSD < 6.73%), stability (RSD < 5.63%), inter- and intra-day precision (RSD < 5.57%), recovery (93.76-111.14%), and the limit of detection (LOD) and limit of quantification (LOQ) varied between 0.45-2.37 ng and 0.96-4.71 ng, respectively. We applied it to the content determination of 16 ginsenosides simultaneously from 28 different ginseng-containing CPMs, which unveiled the ginsenoside content difference among the tested CPMs, and gave useful information to discriminate ginseng in the preparation samples, as well. The MHC-2DLC/ CAD approach exhibited advantages of high specificity, good separation ability, and relative high analysis efficiency, which also justified the feasibility of our proposed ' Monomethod Characterization of Structure Analogs '' strategy in quality evaluation of diverse CPMs that contained different ginseng. |
| WOS关键词 | MASS-SPECTROMETRY ; SEPARATION |
| 资助项目 | Tianjin Committee of Science and Technology of China[22ZYJDSS00040] ; Science & Technology Program of Haihe Laboratory of Modern Chinese Medicine[22HHZYSS00012] ; Science & Technology Program of Haihe Laboratory of Modern Chinese Medicine[22HHZYJC00002] ; National Natural Science Foundation of China[81872996] ; Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine[ZYYCXTD-D-202002] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001077038900001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/307263]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Guo, De-an; Yang, Wen-zhi |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, 501 Haike Rd, Shanghai 201203, Peoples R China 2.Tianjin Univ Tradit Chinese Med, Coll Pharmaceut Engn Tradit Chinese Med, Tianjin 301617, Peoples R China 3.Tianjin Univ Tradit Chinese Med, Haihe Lab Modern Chinese Med, 10 Poyanghu Rd, Tianjin 301617, Peoples R China 4.Tianjin Univ Tradit Chinese Med, Natl Key Lab Chinese Med Modernizat, State Key Lab Component Based Chinese Med, 10 Poyanghu Rd, Tianjin 301617, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Xiao-yan,Jiang, Mei-ting,Wang, Yu,et al. Multiple heart-cutting two-dimensional liquid chromatography/charged aerosol detector assay of ginsenosides for quality evaluation of ginseng from diverse Chinese patent medicines[J]. JOURNAL OF CHROMATOGRAPHY A,2023,1708:10. |
| APA | Xu, Xiao-yan.,Jiang, Mei-ting.,Wang, Yu.,Sun, He.,Jing, Qi.,...&Yang, Wen-zhi.(2023).Multiple heart-cutting two-dimensional liquid chromatography/charged aerosol detector assay of ginsenosides for quality evaluation of ginseng from diverse Chinese patent medicines.JOURNAL OF CHROMATOGRAPHY A,1708,10. |
| MLA | Xu, Xiao-yan,et al."Multiple heart-cutting two-dimensional liquid chromatography/charged aerosol detector assay of ginsenosides for quality evaluation of ginseng from diverse Chinese patent medicines".JOURNAL OF CHROMATOGRAPHY A 1708(2023):10. |
入库方式： OAI收割
来源：上海药物研究所
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