Characterization of ginsenosides from Panax japonicus var. major (Zhu-Zi-Shen) based on ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry and desorption electrospray ionization-mass spectrometry imaging
文献类型：期刊论文
| 作者 | Jiang, Meiting3,4; Li, Xiaohang3,4; Zhao, Yuying3,4; Zou, Yadan3,4; Bai, Maoli3,4; Yang, Zhiming2; Wang, Wei3,4; Xu, Xiaoyan3,4; Wang, Hongda3,4; Yang, Wenzhi2,3,4 |
| 刊名 | CHINESE MEDICINE
 |
| 出版日期 | 2023-09-08 |
| 卷号 | 18期号:1页码:14 |
| ISSN号 | 1749-8546 |
| 关键词 | Panax japonicus var. major Ginsenoside UHPLC/QTOF-MS DESI-MSI Precursor ions list Spatial distribution |
| DOI | 10.1186/s13020-023-00830-9 |
| 通讯作者 | Yang, Wenzhi(wzyang0504@tjutcm.edu.cn) ; Chen, Qinhua(cqh77@163.com) |
| 英文摘要 | Background Panax japonicus var. major (PJM) belongs to the well-known ginseng species used in west China for hundreds of years, which has the effects of lung tonifying and yin nourishing, and exerts the analgesic, antitussive, and hemostatic activities. Compared with the other Panax species, the chemical composition and the spatial tissue distribution of the bioactive ginsenosides in PJM have seldom been investigated. Methods Ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/ QTOF-MS) and desorption electrospray ionization-mass spectrometry imaging (DESI-MSI) were integrated for the systematic characterization and spatial tissue distribution studies of ginsenosides in the rhizome of PJM. Considering the great difficulty in exposing the minor saponins, apart from the conventional Auto MS/MS (M1), two different precursor ions list-including data-dependent acquisition (PIL-DDA) approaches, involving the direct input of an in-house library containing 579 known ginsenosides (M2) and the inclusion of the target precursors screened from the MS1 data by mass defect filtering (M3), were developed. The in situ spatial distribution of various ginsenosides in PJM was profiled based on DESI-MSI with a mass range of m/z 100-1500 in the negative ion mode, with the imaging data processed by the High Definition Imaging (HDI) software. Results Under the optimized condition, 272 ginsenosides were identified or tentatively characterized, and 138 thereof were possibly not ever reported from the Panax genus. They were composed by 75 oleanolic acid type, 22 protopanaxadiol type, 52 protopanaxatriol type, 16 octillol type, 19 malonylated, 35 C-17 side- chain varied, and 53 others. In addition, the DESI-MSI experiment unveiled the differentiated distribution of saponins, but the main location in the cork layer and phloem of the rhizome. The abundance of the oleanolic acid ginsenosides was high in the rhizome slice of PJM, which was consistent with the results obtained by UHPLC/QTOF-MS. Conclusion Comprehensive characterization of the ginsenosides in the rhizome of PJM was achieved, with a large amount of unknown structures unveiled primarily. We, for the first time, reported the spatial tissue distribution of different subtypes of ginsenosides in the rhizome slice of PJM. These results can benefit the quality control and further development of PJM and the other ginseng species. |
| WOS关键词 | GINSENG |
| 资助项目 | Not applicable. |
| WOS研究方向 | Integrative & Complementary Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| 出版者 | BMC |
| WOS记录号 | WOS:001064375800001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/307285]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Yang, Wenzhi; Chen, Qinhua |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Mat Med, 501 Haike Rd, Shanghai 201203, Peoples R China 2.Shenzhen Baoan Authent TCM Therapy Hosp, Shenzhen 518101, Peoples R China 3.Tianjin Univ Tradit Chinese Med, Haihe Lab Modern Chinese Med, 10 Poyanghu Rd, Tianjin 301617, Peoples R China 4.Tianjin Univ Tradit Chinese Med, Natl Key Lab Chinese Med Modernizat, State Key Lab Component Based Chinese Med, 10 Poyanghu Rd, Tianjin 301617, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Meiting,Li, Xiaohang,Zhao, Yuying,et al. Characterization of ginsenosides from Panax japonicus var. major (Zhu-Zi-Shen) based on ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry and desorption electrospray ionization-mass spectrometry imaging[J]. CHINESE MEDICINE,2023,18(1):14. |
| APA | Jiang, Meiting.,Li, Xiaohang.,Zhao, Yuying.,Zou, Yadan.,Bai, Maoli.,...&Guo, Dean.(2023).Characterization of ginsenosides from Panax japonicus var. major (Zhu-Zi-Shen) based on ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry and desorption electrospray ionization-mass spectrometry imaging.CHINESE MEDICINE,18(1),14. |
| MLA | Jiang, Meiting,et al."Characterization of ginsenosides from Panax japonicus var. major (Zhu-Zi-Shen) based on ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry and desorption electrospray ionization-mass spectrometry imaging".CHINESE MEDICINE 18.1(2023):14. |
入库方式： OAI收割
来源：上海药物研究所
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