An integrative profiling of metabolome and transcriptome in the plasma and skeletal muscle following an exercise intervention in diet-induced obese mice
文献类型：期刊论文
| 作者 | Han, Shuang7,8,9; Wu, Qingqian6,8,9; Wang, Mengying5; Yang, Miqi8,9; Sun, Chen3,4; Liang, Jiaqi3,4; Guo, Xiaozhen2; Zhang, Zheyu8,9; Xu, Jingya8,9; Qiu, Xinyuan1 |
| 刊名 | JOURNAL OF MOLECULAR CELL BIOLOGY
 |
| 出版日期 | 2023-08-03 |
| 卷号 | 15期号:3页码:14 |
| ISSN号 | 1674-2788 |
| 关键词 | type 2 diabetes exercise intervention metabolomics transcriptomics |
| DOI | 10.1093/jmcb/mjad016 |
| 通讯作者 | Gao, Yue(gy9821@sina.com) ; Meng, Zhuo-Xian(zxmeng@zju.edu.cn) |
| 英文摘要 | Exercise intervention at the early stage of type 2 diabetes mellitus (T2DM) can aid in the maintenance of blood glucose homeostasis and prevent the development of macrovascular and microvascular complications. However, the exercise-regulated pathways that prevent the development of T2DM remain largely unclear. In this study, two forms of exercise intervention, treadmill training and voluntary wheel running, were conducted for high-fat diet (HFD)-induced obese mice. We observed that both forms of exercise intervention alleviated HFD-induced insulin resistance and glucose intolerance. Skeletal muscle is recognized as the primary site for postprandial glucose uptake and for responsive alteration beyond exercise training. Metabolomic profiling of the plasma and skeletal muscle in Chow, HFD, and HFD-exercise groups revealed robust alterations in metabolic pathways by exercise intervention in both cases. Overlapping analysis identified nine metabolites, including beta-alanine, leucine, valine, and tryptophan, which were reversed by exercise treatment in both the plasma and skeletal muscle. Transcriptomic analysis of gene expression profiles in the skeletal muscle revealed several key pathways involved in the beneficial effects of exercise on metabolic homeostasis. In addition, integrative transcriptomic and metabolomic analyses uncovered strong correlations between the concentrations of bioactive metabolites and the expression levels of genes involved in energy metabolism, insulin sensitivity, and immune response in the skeletal muscle. This work established two models of exercise intervention in obese mice and provided mechanistic insights into the beneficial effects of exercise intervention on systemic energy homeostasis. |
| WOS关键词 | INSULIN SENSITIVITY ; SIGNALING PATHWAYS ; GENE-EXPRESSION ; AMINO-ACIDS ; RESISTANCE ; IMPACT ; RECONSTRUCTION ; PERFORMANCE ; MECHANISMS ; RECOVERY |
| 资助项目 | The authors thank the Meng Lab members for their helpful discussion and technical support to this study. We also thank the Core Facilities of Zhejiang University School of Medicine for technical support. |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:001081418500001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/307354]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Gao, Yue; Meng, Zhuo-Xian |
| 作者单位 | 1.Natl Univ Def Technol, Coll Liberal Arts & Sci, Dept Biol & Chem, Changsha 410073, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 3.China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing 211198, Peoples R China 4.China Pharmaceut Univ, State Key Lab Nat Med, Nanjing 211198, Peoples R China 5.Zhejiang Univ, Sch Med, Sch Publ Hlth, Dept Big Datain Hlth Sci, Hangzhou 310003, Peoples R China 6.Zhejiang Univ, Sch Med, Key Lab Dis Prote Zhejiang Prov, Hangzhou 310058, Peoples R China 7.Zhejiang Univ, Affiliated Hangzhou Peoples Hosp 1, Sch Med, Dept Geriatr, Hangzhou 310006, Peoples R China 8.Zhejiang Univ, Affiliated Hosp 2, Sch Med, Dept Cardiol, Hangzhou 310058, Peoples R China 9.Zhejiang Univ, Affiliated Hosp 2, Sch Med, Dept Pathol & Pathophysiol, Hangzhou 310058, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Shuang,Wu, Qingqian,Wang, Mengying,et al. An integrative profiling of metabolome and transcriptome in the plasma and skeletal muscle following an exercise intervention in diet-induced obese mice[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2023,15(3):14. |
| APA | Han, Shuang.,Wu, Qingqian.,Wang, Mengying.,Yang, Miqi.,Sun, Chen.,...&Meng, Zhuo-Xian.(2023).An integrative profiling of metabolome and transcriptome in the plasma and skeletal muscle following an exercise intervention in diet-induced obese mice.JOURNAL OF MOLECULAR CELL BIOLOGY,15(3),14. |
| MLA | Han, Shuang,et al."An integrative profiling of metabolome and transcriptome in the plasma and skeletal muscle following an exercise intervention in diet-induced obese mice".JOURNAL OF MOLECULAR CELL BIOLOGY 15.3(2023):14. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。