中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
Characterization of natural peptides in Pheretima by integrating proteogenomics and label-free peptidomics

文献类型：期刊论文

作者Luo, Xiaoxiao3,4; Bi, Qirui3; Huang, Dongdong2,3; Li, Yun3; Yao, Changliang3; Zhang, Jianqing3; Wei, Wenlong3; Li, Jiayuan3; Li, Zhenwei3; Zhang, Jingxian1
刊名JOURNAL OF PHARMACEUTICAL ANALYSIS
出版日期2023-09-01
卷号13期号:9页码:1070-1079
ISSN号2095-1779
关键词Pheretima Natural peptides Label-free peptidomics Proteogenomics Animal derived medicine
DOI10.1016/j.jpha.2023.06.006
通讯作者Ji, Shen(jishen2013@163.com) ; Wang, Yurong(yurong1987213@163.com) ; Guo, De-an(daguo@simm.ac.cn)
英文摘要Pheretima, also called "earthworms", is a well-known animal-derived traditional Chinese medicine that is extensively used in over 50 Chinese patent medicines (CPMs) in Chinese Pharmacopoeia (2020 edition). However, its zoological origin is unclear, both in the herbal market and CPMs. In this study, a strategy for integrating in-house annotated protein databases constructed from close evolutionary relationship-sourced RNA sequencing data from public archival resources and various sequencing algorithms (restricted search, open search, and de novo) was developed to characterize the phenotype of natural peptides of three major commercial species of Pheretima, including Pheretima aspergillum (PA), Pheretima vulgaris (PV), and Metaphire magna (MM). We identified 10,477 natural peptides in the PA, 7,451 in PV, and 5,896 in MM samples. Five specific signature peptides were screened and then validated using synthetic peptides; these demonstrated robust specificity for the authentication of PA, PV, and MM. Finally, all marker peptides were successfully applied to identify the zoological origins of Brain Heart capsules and Xiaohuoluo pills, revealing the inconsistent Pheretima species used in these CPMs. In conclusion, our integrated strategy could be used for the in-depth characterization of natural peptides of other animal-derived traditional Chinese medicines, especially non-model species with poorly annotated protein databases.(c) 2023 The Authors. Published by Elsevier B.V. on behalf of Xi'an Jiaotong University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
WOS关键词PROTEINS ; IDENTIFICATION ; GENERATION ; BIOMARKERS ; DISCOVERY
资助项目Key Program of the National Natural Science Foundation of China[82130111] ; National Natural Science Foundation of China[81803716] ; National Administration of Traditional Chinese Medicine, China ; SIMM-SHUTCM Traditional Chinese Medicine Innovation Joint Research Program, China[E2G809H]
WOS研究方向Pharmacology & Pharmacy
语种英语
出版者ELSEVIER
WOS记录号WOS:001086618900001
源URL[http://119.78.100.183/handle/2S10ELR8/307551]  
专题中国科学院上海药物研究所
通讯作者Ji, Shen; Wang, Yurong; Guo, De-an
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, NMPA Key Lab Qual Control Tradit Chinese Med, Shanghai 201203, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Res Ctr TCM Standardizat Technol, Shanghai 201203, Peoples R China
4.China Pharmaceut Univ, Sch Basic Med & Clin Pharm, Nanjing 211198, Peoples R China
推荐引用方式
GB/T 7714		 Luo, Xiaoxiao,Bi, Qirui,Huang, Dongdong,et al. Characterization of natural peptides in Pheretima by integrating proteogenomics and label-free peptidomics[J]. JOURNAL OF PHARMACEUTICAL ANALYSIS,2023,13(9):1070-1079.
APA Luo, Xiaoxiao.,Bi, Qirui.,Huang, Dongdong.,Li, Yun.,Yao, Changliang.,...&Guo, De-an.(2023).Characterization of natural peptides in Pheretima by integrating proteogenomics and label-free peptidomics.JOURNAL OF PHARMACEUTICAL ANALYSIS,13(9),1070-1079.
MLA Luo, Xiaoxiao,et al."Characterization of natural peptides in Pheretima by integrating proteogenomics and label-free peptidomics".JOURNAL OF PHARMACEUTICAL ANALYSIS 13.9(2023):1070-1079.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。