中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
SGF29 nuclear condensates reinforce cellular aging

文献类型：期刊论文

作者Yan, Kaowen11,12,13,14; Ji, Qianzhao13,14; Zhao, Dongxin10; Li, Mingheng9,13; Sun, Xiaoyan7,8,13; Wang, Zehua9,13; Liu, Xiaoqian9,11,12,13; Liu, Zunpeng9,13; Li, Hongyu6,13; Ding, Yingjie7,8,13
刊名CELL DISCOVERY
出版日期2023-11-07
卷号9期号:1页码:21
DOI10.1038/s41421-023-00602-7
通讯作者Qu, Jing(qujing@ioz.ac.cn) ; Zhang, Weiqi(zhangwq@big.ac.cn) ; Liu, Guang-Hui(ghliu@ioz.ac.cn)
英文摘要Phase separation, a biophysical segregation of subcellular milieus referred as condensates, is known to regulate transcription, but its impacts on physiological processes are less clear. Here, we demonstrate the formation of liquid-like nuclear condensates by SGF29, a component of the SAGA transcriptional coactivator complex, during cellular senescence in human mesenchymal progenitor cells (hMPCs) and fibroblasts. The Arg 207 within the intrinsically disordered region is identified as the key amino acid residue for SGF29 to form phase separation. Through epigenomic and transcriptomic analysis, our data indicated that both condensate formation and H3K4me3 binding of SGF29 are essential for establishing its precise chromatin location, recruiting transcriptional factors and co-activators to target specific genomic loci, and initiating the expression of genes associated with senescence, such as CDKN1A. The formation of SGF29 condensates alone, however, may not be sufficient to drive H3K4me3 binding or achieve transactivation functions. Our study establishes a link between phase separation and aging regulation, highlighting nuclear condensates as a functional unit that facilitate shaping transcriptional landscapes in aging.
WOS关键词TCOF1 GENE-PRODUCT ; PHASE-SEPARATION ; COACTIVATOR COMPLEXES ; DISORDERED PROTEINS ; SAGA ; TRANSCRIPTION ; WERNER ; MODEL ; ATAC ; SENESCENCE
资助项目We are grateful to G. He from Institute of Zoology, Chinese Academy of Sciences for instruction in the use of the microscope. We are also grateful to J. Wang from Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences for LC-MS/MS a[2022YFA1103700] ; We are grateful to G. He from Institute of Zoology, Chinese Academy of Sciences for instruction in the use of the microscope. We are also grateful to J. Wang from Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences for LC-MS/MS a[2020YFA0804000] ; We are grateful to G. He from Institute of Zoology, Chinese Academy of Sciences for instruction in the use of the microscope. We are also grateful to J. Wang from Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences for LC-MS/MS a[2020YFA0112200] ; We are grateful to G. He from Institute of Zoology, Chinese Academy of Sciences for instruction in the use of the microscope. We are also grateful to J. Wang from Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences for LC-MS/MS a[2021YFF1201005] ; We are grateful to G. He from Institute of Zoology, Chinese Academy of Sciences for instruction in the use of the microscope. We are also grateful to J. Wang from Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences for LC-MS/MS a[2019YFA0110100] ; We are grateful to G. He from Institute of Zoology, Chinese Academy of Sciences for instruction in the use of the microscope. We are also grateful to J. Wang from Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences for LC-MS/MS a[2021ZD0202400] ; National Key Ramp;D Program of China[81921006] ; National Key Ramp;D Program of China[82125011] ; National Key Ramp;D Program of China[92149301] ; National Key Ramp;D Program of China[92168201] ; National Key Ramp;D Program of China[91949209] ; National Key Ramp;D Program of China[92049304] ; National Key Ramp;D Program of China[92049116] ; National Key Ramp;D Program of China[32121001] ; National Key Ramp;D Program of China[82192863] ; National Key Ramp;D Program of China[32171447] ; National Key Ramp;D Program of China[82330044] ; National Key Ramp;D Program of China[82122024] ; National Key Ramp;D Program of China[82071588] ; National Natural Science Foundation of China[XDA16000000] ; Strategic Priority Research Program of the Chinese Academy of Sciences[Z190019] ; Program of the Beijing Natural Science Foundation[YSBR-076] ; Program of the Beijing Natural Science Foundation[YSBR-012] ; CAS Project for Young Scientists in Basic Research[E1CAZW0401] ; Youth Innovation Promotion Association of CAS[CAS-WX2021SF-0301] ; Youth Innovation Promotion Association of CAS[CAS-WX2022SDC-XK14] ; Youth Innovation Promotion Association of CAS[CAS-WX2021SF-0101] ; Informatization Plan of Chinese Academy of Sciences[2021-1045] ; New Cornerstone Science Foundation[12300927] ; Excellent Young Talents Program of Capital Medical University[11000023T000002036310] ; Project for Technology Development of Beijing-affiliated Medical Research Institutes[BPHR202203105] ; Excellent Young Talents Training Program for the Construction of Beijing Municipal University Teacher Team
WOS研究方向Cell Biology
语种英语
出版者SPRINGERNATURE
WOS记录号WOS:001096376500001
源URL[http://119.78.100.183/handle/2S10ELR8/307637]  
专题中国科学院上海药物研究所
通讯作者Qu, Jing; Zhang, Weiqi; Liu, Guang-Hui
作者单位1.Univ Chinese Acad Sci, Sino Danish Coll, Beijing, Peoples R China
2.Altos Labs Inc, San Diego, CA USA
3.Capital Med Univ, Xuanwu Hosp, Aging Translat Med Ctr, Int Ctr Aging & Canc, Beijing, Peoples R China
4.Capital Med Univ, Xuanwu Hosp, Natl Clin Res Ctr Geriatr Disorders, Beijing, Peoples R China
5.Capital Med Univ, Xuanwu Hosp, Adv Innovat Ctr Human Brain Protect, Beijing, Peoples R China
6.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromolecules, Natl Lab Biomacromolecules, Beijing, Peoples R China
7.China Natl Ctr Bioinformat, Beijing, Peoples R China
8.Beijing Inst Genom, Chinese Acad Sci, CAS Key Lab Genom & Precis Med, Beijing, Peoples R China
9.Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing, Peoples R China
10.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China
推荐引用方式
GB/T 7714		 Yan, Kaowen,Ji, Qianzhao,Zhao, Dongxin,et al. SGF29 nuclear condensates reinforce cellular aging[J]. CELL DISCOVERY,2023,9(1):21.
APA Yan, Kaowen.,Ji, Qianzhao.,Zhao, Dongxin.,Li, Mingheng.,Sun, Xiaoyan.,...&Liu, Guang-Hui.(2023).SGF29 nuclear condensates reinforce cellular aging.CELL DISCOVERY,9(1),21.
MLA Yan, Kaowen,et al."SGF29 nuclear condensates reinforce cellular aging".CELL DISCOVERY 9.1(2023):21.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。