Global Profiling of Lysine Acetylation and Lactylation in Kupffer Cells
文献类型：期刊论文
| 作者 | Sung, Eunji5; Sim, Hyunchae4; Cho, Young-Chang3; Lee, Wonhwa2; Bae, Jong-Sup5; Tan, Minjia1 ; Lee, Sangkyu4
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| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2023-10-28 |
| 页码 | 9 |
| 关键词 | global proteomics Kupffer cells lysine acetylation lysine lactylation proteinmodification |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/acs.jproteome.3c00156 |
| 通讯作者 | Lee, Sangkyu(sangkyu@skku.edu) |
| 英文摘要 | Among the various cell types that constitute the liver, Kupffer cells (KCs) are responsible for the elimination of gut-derived foreign products. Protein lysine acetylation (Kac) and lactylation (Kla) are dynamic and reversible post-translational modifications, and various global acylome studies have been conducted for liver and liver-derived cells. However, no such studies have been conducted on KCs. In this study, we identified 2198 Kac sites in 925 acetylated proteins and 289 Kla sites in 181 lactylated proteins in immortalized mouse KCs using global acylome technology. The subcellular distributions of proteins with Kac and Kla site modifications differed. Similarly, the specific sequence motifs surrounding acetylated or lactylated lysine residues also showed differences. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to better understand the differentially expressed proteins in the studies by Kac and Kla. In the newly identified Kla, we found K82 lactylation in the high-mobility group box-1 protein in the neutrophil extracellular trap formation category using KEGG enrichment analyses. Here, we report the first proteomic survey of Kac and Kla in KCs. |
| WOS关键词 | GROUP BOX 1 ; METABOLIC-REGULATION ; PROTEOMIC ANALYSIS ; PROTEIN ; ACETYLOME ; SIRT3 |
| 资助项目 | National Research Foundation of Korea[2021K2A9A2A06044011] ; National Research Foundation of Korea[2021R1A2C1003510] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001096758400001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/307718]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Lee, Sangkyu |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Sungkyunkwan Univ, Dept Chem, Suwon 16419, South Korea 3.Chonnam Natl Univ, Coll Pharm, Gwangju 61186, South Korea 4.Sungkyunkwan Univ, Sch Pharm, Suwon 16419, South Korea 5.Kyungpook Natl Univ, Coll Pharm, Daegu 41566, South Korea |
| 推荐引用方式 GB/T 7714 | Sung, Eunji,Sim, Hyunchae,Cho, Young-Chang,et al. Global Profiling of Lysine Acetylation and Lactylation in Kupffer Cells[J]. JOURNAL OF PROTEOME RESEARCH,2023:9. |
| APA | Sung, Eunji.,Sim, Hyunchae.,Cho, Young-Chang.,Lee, Wonhwa.,Bae, Jong-Sup.,...&Lee, Sangkyu.(2023).Global Profiling of Lysine Acetylation and Lactylation in Kupffer Cells.JOURNAL OF PROTEOME RESEARCH,9. |
| MLA | Sung, Eunji,et al."Global Profiling of Lysine Acetylation and Lactylation in Kupffer Cells".JOURNAL OF PROTEOME RESEARCH (2023):9. |
入库方式： OAI收割
来源：上海药物研究所
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