A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation
文献类型：期刊论文
| 作者 | Guo, Na2,4; Liu, Yuan1,4; Hao, Qiang2,4; Sun, Mingzhe1,4 ; Li, Fuhua1,3,4
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| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 出版日期 | 2023-07-01 |
| 卷号 | 24期号:13页码:19 |
| 关键词 | mannose receptor Litopenaeus vannamei pathogen recognition inflammation ROS |
| ISSN号 | 1661-6596 |
| DOI | 10.3390/ijms241310665 |
| 通讯作者 | Liu, Yuan(liuyuan@qdio.ac.cn) |
| 英文摘要 | Mannose receptor, as a member of the C-type lectin superfamily, is a non-canonical pattern recognition receptor that can internalize pathogen-associated ligands and activate intracellular signaling. Here, a mannose receptor gene, LvMR, was identified from the Pacific white shrimp Litopenaeus vannamei. LvMR encoded a signal peptide, a fibronectin type II (FN II) domain, and two carbohydrate-recognition domains (CRDs) with special EPS and FND motifs. LvMR transcripts were mainly detected in the hepatopancreas, and presented a time-dependent response after pathogen challenge. The recombinant LvMR (rLvMR) could bind to various PAMPs and agglutinate microorganisms in a Ca2+-dependent manner with strong binding ability to (D)-mannose and N-acetyl sugars. The knockdown of LvMR enhanced the expression of most NF-& kappa;B pathway genes, inflammation and redox genes, while it had no obvious effect on the transcription of most phagocytosis genes. Moreover, the knockdown of LvMR caused an increase in reactive oxygen species (ROS) content and inducible nitric oxide synthase (iNOS) activity in the hepatopancreas after Vibrio parahaemolyticus infection. All these results indicate that LvMR might perform as a PRR in immune recognition and a negative regulator of inflammation during bacterial infection. |
| WOS关键词 | C-TYPE-LECTIN ; MOLECULAR-CLONING ; SHRIMP ; IDENTIFICATION ; HEMOCYTES ; CRD |
| 资助项目 | Key Program of National Natural Science Foundation of China[31830100] ; National Natural Science Foundations of China[32273172] ; Taishan Scholars Program ; [CARS-48] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001030955700001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/182499]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Yuan |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 2.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China 3.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Na,Liu, Yuan,Hao, Qiang,et al. A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2023,24(13):19. |
| APA | Guo, Na,Liu, Yuan,Hao, Qiang,Sun, Mingzhe,&Li, Fuhua.(2023).A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,24(13),19. |
| MLA | Guo, Na,et al."A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24.13(2023):19. |
入库方式： OAI收割
来源：海洋研究所
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