Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)
文献类型：期刊论文
| 作者 | Wang, Xueying1,2 ; Zhao, Ning1,2,3; Wang, Tao1,2,4; Huang, Jinwei1,2,3; Liu, Qinghua1,2; Li, Jun1,2
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| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 出版日期 | 2023-07-01 |
| 卷号 | 24期号:14页码:12 |
| ISSN号 | 1661-6596 |
| 关键词 | turbot aqps spermatogenesis sperm |
| DOI | 10.3390/ijms241411770 |
| 通讯作者 | Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn) |
| 英文摘要 | The development and maturation of sperm entails intricate metabolic processes involving water molecules, amino acids, hormones, and various substances. Among these processes, the role of aquaporins (aqps) in the testis is crucial. Turbot (Scophthalmus maximus) is a significant marine flatfish species in China; however, natural egg laying in females is not feasible under cultured conditions. Consequently, artificial insemination becomes necessary, requiring the retrieval of sperm and eggs through artificial methods. In this study, we combined genomic, transcriptomics, RT-qPCR, computer-assisted sperm analysis (CASA), and immunohistochemistry to investigate the involvement of the aqp family in spermatogenesis in turbot. Through genomic data analysis, we identified 16 aqps genes dispersed across 13 chromosomes, each exhibiting the characteristic major intrinsic protein (MIP) domain associated with AQPs. The results from RNA-seq and RT-qPCR analysis revealed prominent expression of aqp4, 10, and 12 during the proliferative stage, whereas aqp1 showed primary expression during the mature stage. aqp11 displayed high expression levels during both MSII and MSV stages, potentially contributing significantly to the proliferation and maturation of male germ cells. Conversely, aqp8 showed elevated expression levels during the MSIII, MSIII-IV, and MSIV stages, suggesting its direct involvement in spermiogenesis. Immunohistochemical analysis unveiled the predominant localization of AQP1 protein in male germ cells rather than Sertoli cells, specifically concentrated in the head of sperm within cysts. Furthermore, a noteworthy decline in sperm motility was observed when sperm were subjected to treatment with either the AQP1-specific inhibitor (HgCl2) or the AQP1 antibody. However, no direct correlation was found between the expression of Smaqp1 and sperm quality. Overall, these findings provide new insights into the involvement of aqps in teleost spermatogenesis. Moreover, they hold potential for improving techniques related to sperm activation and cryopreservation, offering valuable knowledge for future advancements in this field. |
| WOS关键词 | PHYSIOLOGICAL VOLUME REGULATION ; SPERM MOTILITY ; EXPRESSION ; CHANNELS ; AQUAPORINS ; TESTIS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:001036142300001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/182574]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Qinghua; Li, Jun |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xueying,Zhao, Ning,Wang, Tao,et al. Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2023,24(14):12. |
| APA | Wang, Xueying,Zhao, Ning,Wang, Tao,Huang, Jinwei,Liu, Qinghua,&Li, Jun.(2023).Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,24(14),12. |
| MLA | Wang, Xueying,et al."Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24.14(2023):12. |
入库方式： OAI收割
来源：海洋研究所
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