The Responses of Alternative Splicing during Heat Stress in the Pacific White Shrimp Litopenaeus vannamei
文献类型：期刊论文
| 作者 | Zhang, Xiaoxi1,2,3,4 ; Zhang, Xiaojun1,2,3,4; Yuan, Jianbo1,2,3,4; Li, Fuhua1,2,3,4
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| 刊名 | GENES
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| 出版日期 | 2023-07-01 |
| 卷号 | 14期号:7页码:12 |
| 关键词 | alternative splicing heat stress Litopenaeus vannamei RNA-Seq |
| DOI | 10.3390/genes14071473 |
| 通讯作者 | Li, Fuhua(fhli@qdio.ac.cn) |
| 英文摘要 | Heat tolerance is increasingly becoming a crucial trait for aquaculture species in the face of rapidly changing climate conditions. Alternative splicing (AS) is a vital mechanism within cells that modulates gene abundance and functional diversity, enabling organisms to effectively respond to diverse stressful conditions, including thermal stress. However, it is still uncertain whether AS contributes to heat tolerance in shrimp. In this study, we conducted an extensive transcriptome analysis on the Pacific white shrimp, Litopenaeus vannamei, revealing a total of 1267, 987, and 130 differential AS events (DAS) in the gill, hepatopancreas, and muscle, respectively, following exposure to heat stress. Among all of the DAS events, exon skipping (ES) was the predominant form of splicing modification observed. Interestingly, a minor portion of DAS genes exhibited overlap across the three tissues, implying that heat stress exerts unique effects on various tissue types. Moreover, the functional enrichment analysis demonstrated that commonly identified DAS genes were primarily associated with the "spliceosome" pathway, indicating that the AS of splicing-related genes played a crucial role in the response to heat stress. Our findings also revealed that heat stress tended to induce longer mRNA isoforms through differential alternative 3 & PRIME; splice site (A3SS) events. Notably, A3SS events exhibited the highest proportion of maintained open reading frames (ORFs) under heat stress. Interestingly, we observed a limited overlap between the genes exhibiting DAS and those showing differential gene expression (DEG), indicating that AS may function as a distinct regulatory mechanism independent of transcriptional regulation in response to heat stress. This is the first comprehensive study on AS in crustacea species under heat stress, which broadens our understanding of the regulatory mechanisms governing the crustaceans' response to environmental stress, providing valuable insights for the aquaculture breeding of shrimp and other aquatic animals. |
| WOS关键词 | GENOME-WIDE ANALYSIS ; TRANSCRIPTOME ; ADAPTATION ; MECHANISMS ; EXPRESSION ; PROTEINS |
| 资助项目 | National Natural Science Foundation of China[42206091] ; National Natural Science Foundation of China[31972782] ; National Natural Science Foundation of China[42176105] ; China Postdoctoral Science Foundation[2021M703249] ; Agriculture Research System-48 of China[CARS-48] ; Taishan Scholars Program |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:001038500600001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/182595]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Fuhua |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xiaoxi,Zhang, Xiaojun,Yuan, Jianbo,et al. The Responses of Alternative Splicing during Heat Stress in the Pacific White Shrimp Litopenaeus vannamei[J]. GENES,2023,14(7):12. |
| APA | Zhang, Xiaoxi,Zhang, Xiaojun,Yuan, Jianbo,&Li, Fuhua.(2023).The Responses of Alternative Splicing during Heat Stress in the Pacific White Shrimp Litopenaeus vannamei.GENES,14(7),12. |
| MLA | Zhang, Xiaoxi,et al."The Responses of Alternative Splicing during Heat Stress in the Pacific White Shrimp Litopenaeus vannamei".GENES 14.7(2023):12. |
入库方式： OAI收割
来源：海洋研究所
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