EAT-2 attenuates C. elegans development via metabolic remodeling in a chemically defined food environment
文献类型：期刊论文
| 作者 | Cao, Xuwen1,2,3,5,6 ; Xie, Yusu1,5,6; Yang, Hanwen1,5,6; Sun, Peiqi1,3,5,6; Xue, Beining1,3,5,6; Garcia, L. Rene4; Zhang, Liusuo1,5,6
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| 刊名 | CELLULAR AND MOLECULAR LIFE SCIENCES
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| 出版日期 | 2023-08-01 |
| 卷号 | 80期号:8页码:18 |
| ISSN号 | 1420-682X |
| 关键词 | C. elegans maintenance medium CeMM Development Acetylcholine receptor Fatty acid C17ISO S-adenosylmethionine SAM |
| DOI | 10.1007/s00018-023-04849-x |
| 通讯作者 | Zhang, Liusuo(lzhang@qdio.ac.cn) |
| 英文摘要 | Dietary intake and nutrient composition regulate animal growth and development; however, the underlying mechanisms remain elusive. Our previous study has shown that either the mammalian deafness homolog gene tmc-1 or its downstream acetylcholine receptor gene eat-2 attenuates Caenorhabditis elegans development in a chemically defined food CeMM (C. elegans maintenance medium) environment, but the underpinning mechanisms are not well-understood. Here, we found that, in CeMM food environment, for both eat-2 and tmc-1 fast-growing mutants, several fatty acid synthesis and elongation genes were highly expressed, while many fatty acid beta-oxidation genes were repressed. Accordingly, dietary supplementation of individual fatty acids, such as monomethyl branch chain fatty acid C17ISO, palmitic acid and stearic acid significantly promoted wild-type animal development on CeMM, and mutations in either C17ISO synthesis gene elo-5 or elo-6 slowed the rapid growth of eat-2 mutant. Tissue-specific rescue experiments showed that elo-6 promoted animal development mainly in the intestine. Furthermore, transcriptome and metabolome analyses revealed that elo-6/C17ISO regulation of C. elegans development may be correlated with up-regulating expression of cuticle synthetic and hedgehog signaling genes, as well as promoting biosynthesis of amino acids, amino acid derivatives and vitamins. Correspondingly, we found that amino acid derivative S-adenosylmethionine and its upstream metabolite methionine sulfoxide significantly promoted C. elegans development on CeMM. This study demonstrated that C17ISO, palmitic acid, stearic acid, S-adenosylmethionine and methionine sulfoxide inhibited or bypassed the TMC-1 and EAT-2-mediated attenuation of development via metabolic remodeling, and allowed the animals to adapt to the new nutritional niche. |
| WOS关键词 | CHAIN FATTY-ACID ; CAENORHABDITIS-ELEGANS ; DIET ; INSULIN ; IDENTIFICATION ; BIOSYNTHESIS ; LONGEVITY ; EXTENSION ; PATHWAYS ; CIRCUIT |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| 出版者 | SPRINGER BASEL AG |
| WOS记录号 | WOS:001030563000003 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/182634]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Zhang, Liusuo |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Shandong Univ, Inst Marine Sci & Technol, 72 Binhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA 5.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Xuwen,Xie, Yusu,Yang, Hanwen,et al. EAT-2 attenuates C. elegans development via metabolic remodeling in a chemically defined food environment[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2023,80(8):18. |
| APA | Cao, Xuwen.,Xie, Yusu.,Yang, Hanwen.,Sun, Peiqi.,Xue, Beining.,...&Zhang, Liusuo.(2023).EAT-2 attenuates C. elegans development via metabolic remodeling in a chemically defined food environment.CELLULAR AND MOLECULAR LIFE SCIENCES,80(8),18. |
| MLA | Cao, Xuwen,et al."EAT-2 attenuates C. elegans development via metabolic remodeling in a chemically defined food environment".CELLULAR AND MOLECULAR LIFE SCIENCES 80.8(2023):18. |
入库方式： OAI收割
来源：海洋研究所
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