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Mechanisms of nucleic acid degradation and high hydrostatic pressure tolerance of a novel deep-sea wall-less bacterium

文献类型：期刊论文

作者Zheng, Rikuan1,2,3,4; Wang, Chong1,2,3,4; Cai, Ruining1,2,3,4,5; Shan, Yeqi1,2,3,4,5; Sun, Chaomin1,2,3,4,5
刊名MBIO
出版日期2023-08-08
页码19
关键词deep sea wall-less bacteria nucleic acid degradation high pressure tolerance chronic bacteriophage
ISSN号2150-7511
DOI10.1128/mbio.00958-23
通讯作者Sun, Chaomin(sunchaomin@qdio.ac.cn)
英文摘要Wall-less bacteria are broadly distributed in diverse habitats. They evolved from a common ancestor within the Firmicutes phylum through reductive evolution. Here, we report the cultivation, characterization, and polyphasic taxonomic analysis of the novel free-living wall-less bacterium, Hujiaoplasma nucleasis zrk29. We demonstrated that strain zrk29 had a strong ability to degrade DNA and RNA both under laboratory conditions and in the deep sea. We found that nucleic acids induced strain zrk29 to release chronic bacteriophages which supported strain zrk29 and other marine bacteria to metabolize nucleic acids without lysing host cells. We also showed that strain zrk29 tolerated high hydrostatic pressure via two pathways: (i) by transporting cations into its cells to increase intracellular osmotic pressure and (ii) by adjusting the unsaturated fatty acid chain content in its cell membrane phospholipids to increase cell membrane fluidity. This study extends our understanding of free-living wall-less bacteria and provides a useful model to explore the unique adaptation mechanisms of deep-sea microbes. IMPORTANCEThe unique physiology and survival strategies of the Tenericutes bacterium-a typical wall-less bacterium-have fascinated scientists and the public, especially in extreme deep-sea environments where there is high hydrostatic pressure (HHP) and limited availability of nutrients. Here, we have isolated a novel free-living Tenericutes strain from deep-sea sediment and have found that it metabolizes nucleic acids with the support of chronic bacteriophages. This Tenericutes strain tolerates HHP stress by increasing intracellular osmotic pressure and the unsaturated fatty acid chain content of phospholipids in its cell membrane. Our results provide insights into the unique physiology of deep-sea free-living Tenericutes bacteria and highlight the significant role that chronic bacteriophages play in assisting wall-less bacteria to adapt to harsh conditions. The unique physiology and survival strategies of the Tenericutes bacterium-a typical wall-less bacterium-have fascinated scientists and the public, especially in extreme deep-sea environments where there is high hydrostatic pressure (HHP) and limited availability of nutrients. Here, we have isolated a novel free-living Tenericutes strain from deep-sea sediment and have found that it metabolizes nucleic acids with the support of chronic bacteriophages. This Tenericutes strain tolerates HHP stress by increasing intracellular osmotic pressure and the unsaturated fatty acid chain content of phospholipids in its cell membrane. Our results provide insights into the unique physiology of deep-sea free-living Tenericutes bacteria and highlight the significant role that chronic bacteriophages play in assisting wall-less bacteria to adapt to harsh conditions.
WOS关键词EXTRACELLULAR DNA ; MOLECULAR-BIOLOGY ; GENOMICS ; 2FE-2S ; IMPACT
资助项目National Natural Technology Innovation Project[42206107] ; MOST | National Natural Science Foundation of China (NSFC)[92051107]
WOS研究方向Microbiology
语种英语
WOS记录号WOS:001044090300001
出版者AMER SOC MICROBIOLOGY
源URL[http://ir.qdio.ac.cn/handle/337002/182711]  
专题海洋研究所_实验海洋生物学重点实验室
海洋研究所_深海极端环境与生命过程研究中心
通讯作者Sun, Chaomin
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Shandong, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Shandong, Peoples R China
5.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Zheng, Rikuan,Wang, Chong,Cai, Ruining,et al. Mechanisms of nucleic acid degradation and high hydrostatic pressure tolerance of a novel deep-sea wall-less bacterium[J]. MBIO,2023:19.
APA Zheng, Rikuan,Wang, Chong,Cai, Ruining,Shan, Yeqi,&Sun, Chaomin.(2023).Mechanisms of nucleic acid degradation and high hydrostatic pressure tolerance of a novel deep-sea wall-less bacterium.MBIO,19.
MLA Zheng, Rikuan,et al."Mechanisms of nucleic acid degradation and high hydrostatic pressure tolerance of a novel deep-sea wall-less bacterium".MBIO (2023):19.

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来源：海洋研究所

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