Expression characteristics of Hsd3b7 in the gonads of Paralichthys olivaceus
文献类型：期刊论文
| 作者 | Zou, Congcong1,2,3 ; Wang, Wenxiang1,2,3; Shu, Chang1,2,3; Liang, Shaoshuai2,3; Zou, Yuxia2,3; Wang, Lijuan2,3; Wu, Zhihao2,3; Liu, Yan2,3; You, Feng2,3
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| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
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| 出版日期 | 2023-06-01 |
| 卷号 | 266页码:10 |
| ISSN号 | 1096-4959 |
| 关键词 | Flounder Hsd3b7 Expression Gonadal differentiation and development Sox9a |
| DOI | 10.1016/j.cbpb.2023.110848 |
| 通讯作者 | You, Feng(youfeng@qdio.ac.cn) |
| 英文摘要 | Steroidogenesis is an important biological process for gonadal differentiation and development. In mammals, 38hydroxysteroid dehydrogenase 7 (HSD3B7) could convert 38-hydroxy of 7 & alpha;-hydroxycholesterol into a ketone and form 7 & alpha;-hydroxy-4-cholesten-3-one, which may affect steroidogenesis. However, in fish, the study of Hsd3b7 is still lacking. In this study, Hsd3b7 was identified in the olive flounder Paralichthys olivaceus, an important mariculture fish. According to bioinformatics analysis, Hsd3b7 belongs to a Rossmann-fold NAD(P)(+)-binding protein and can interact in a predictable manner with Hsd17b2, -3, and - 4, which play a role in steroidogenesis. In the adult flounder, Hsd3b7 was expressed in various tissues, at particularly high level in male muscle. The expression levels of Hsd3b7 at gonadal development stages I-V initially increased and then decreased, with an inflection point in the ovary at stage III and in the testis at stage IV. At stage III, the expression level of Hsd3b7 was significantly higher in the ovary than in the testis (P < 0.01). The results of in situ hybridization (ISH) revealed that it was mainly expressed in oocytes of phases I-IV or around oocytes of phases IV-V in the ovaries and around spermatid lobules at stages IV-V in the testes. Three regulatory sites of SRY-box transcription factor 9 (Sox9), a transcription factor involved in steroidogenesis and gonadal differentiation, were predicted in the promoter of Hsd3b7. After intraperitoneal injection with the recombination flounder Sox9a, the expression of Hsd3b7 was significantly up-regulated (P < 0.01). During the flounder gonadal differentiation, 178-estradiol (E2, 5 & mu;g/g feed) and 17 & alpha;-methyltestosterone (T, 5 & mu;g/g feed) were used to obtain the phenotypic female or male flounder, and the results showed that in the E2 group, Hsd3b7 expression was highest at 2 cm TL, the primordial gonad stage, which was significantly higher than that at 12 cm TL (P < 0.05). In the T group, Hsd3b7 expression level was also highest at 2 cm TL and significantly higher than at 10 and 12 cm TL (P < 0.05). Moreover, Hsd3b7 was detected to be localized mainly around oogonia and spermatogonia during the differentiated gonads with ISH. These findings first introduce the expression characteristics of Hsd3b7 and the effect of Sox9a on its expression, which contribute to our understanding of the function of Hsd3b7 in fish gonads. |
| WOS关键词 | DIFFERENTIATION ; HORMONE ; TEMMINCK |
| 资助项目 | National Key Research and Devel- opment Program of China[2022YFD2400402] ; National Key Research and Devel- opment Program of China[2018YFD0900202] ; National Natural Science Foundation of China[31872558] ; National Natural Science Foundation of China[31602143] ; National Natural Science Foundation of China[31772834] ; National Marine Genetic Resource Center |
| WOS研究方向 | Biochemistry & Molecular Biology ; Zoology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE INC |
| WOS记录号 | WOS:001041123500001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/182723]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | You, Feng |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zou, Congcong,Wang, Wenxiang,Shu, Chang,et al. Expression characteristics of Hsd3b7 in the gonads of Paralichthys olivaceus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2023,266:10. |
| APA | Zou, Congcong.,Wang, Wenxiang.,Shu, Chang.,Liang, Shaoshuai.,Zou, Yuxia.,...&You, Feng.(2023).Expression characteristics of Hsd3b7 in the gonads of Paralichthys olivaceus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,266,10. |
| MLA | Zou, Congcong,et al."Expression characteristics of Hsd3b7 in the gonads of Paralichthys olivaceus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 266(2023):10. |
入库方式： OAI收割
来源：海洋研究所
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