Downregulation of ferredoxin-NADP plus oxidoreductase enhances oxidized pentose phosphate pathway via NADPH/NADP plus ratio in the strong resurrection intertidal red alga Neopyropia yezoensis with economic value
文献类型：期刊论文
| 作者 | Guo, Menglin1,2,3; Shao, Zhizhuo1,2,3; Zheng, Zhenbing1,2; Wang, Guangce1,2 |
| 刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
 |
| 出版日期 | 2023-07-01 |
| 卷号 | 74页码:9 |
| 关键词 | Ferredoxin-NADP plus oxidoreductase Neopyropia yezoensis Oxidized pentose phosphate pathway Cyclic electron transportation |
| ISSN号 | 2211-9264 |
| DOI | 10.1016/j.algal.2023.103204 |
| 通讯作者 | Zheng, Zhenbing(zbzheng@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn) |
| 英文摘要 | Ferredoxin-NADP+ oxidoreductase (FNR) is involved in reducing Fd in linear electron transportation (LET) in chloroplast. While the oxidized pentose phosphate pathway (OPPP) occurred in cytoplasm has close relationship with cyclic electron transportation (CET) under stress condition. However, whether FNR and OPPP cooperate with each other, and in what way they are associated remains unclear. In this study, Neopyropia yezoensis, a strong resurrection red macroalga, was used to study the relationship between FNR and OPPP. FNR genetic transformation strains of N. yezoensis, fnrOE (overexpression) and fnrSi (silence) were obtained. The results showed that the growth rate of fnrSi was reduced remarkably. The expression level of genes encoding key en-zymes in OPPP and enzyme activity of G6PDH were up regulated in transgenic strains, indicating the enhancement of OPPP. Besides, the NADPH and NADP+ content in WT and transgenic lines were also measured. Y (I) of both fnrOE and fnrSi decreased significantly under dark treatment, indicating that FNR content syner-gistically affected CET pathway with OPPP in N. yezoensis. All the results indicated that changes of FNR content could enhance OPPP, which further promote the CET operation. This is the first time to find the close relationship between photosynthetic electron transportation and carbohydrate metabolism in strong resurrection plant. |
| WOS关键词 | CYCLIC ELECTRON FLOW ; PHOTOSYSTEM-I ; REDUCTASE FNR ; STRESS ; DEHYDROGENASE ; ASSOCIATION ; DRIVES ; GROWTH ; PGR5 |
| 资助项目 | Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program[2022LZGC004] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; earmarked fund for CARS, Technology special project in North Jiangsu Province[SZ-LYG202122] ; earmarked fund for CARS, Technology special project in North Jiangsu Province[CARS-50] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001052196400001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/182798]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Zheng, Zhenbing; Wang, Guangce |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 168 Wenhai Rd, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Menglin,Shao, Zhizhuo,Zheng, Zhenbing,et al. Downregulation of ferredoxin-NADP plus oxidoreductase enhances oxidized pentose phosphate pathway via NADPH/NADP plus ratio in the strong resurrection intertidal red alga Neopyropia yezoensis with economic value[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2023,74:9. |
| APA | Guo, Menglin,Shao, Zhizhuo,Zheng, Zhenbing,&Wang, Guangce.(2023).Downregulation of ferredoxin-NADP plus oxidoreductase enhances oxidized pentose phosphate pathway via NADPH/NADP plus ratio in the strong resurrection intertidal red alga Neopyropia yezoensis with economic value.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,74,9. |
| MLA | Guo, Menglin,et al."Downregulation of ferredoxin-NADP plus oxidoreductase enhances oxidized pentose phosphate pathway via NADPH/NADP plus ratio in the strong resurrection intertidal red alga Neopyropia yezoensis with economic value".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 74(2023):9. |
入库方式： OAI收割
来源：海洋研究所
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