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LysM-containing proteins function in the resistance of Litopenaeus vannamei against Vibrio parahaemolyticus infection

文献类型：期刊论文

作者Miao, Miao1,2,5; Li, Shihao1,4,5; Yu, Yang1,4,5; Li, Fuhua1,3,4,5
刊名DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
出版日期2023-11-01
卷号148页码:9
ISSN号0145-305X
关键词Lysin motif Litopenaeus vannamei Binding activity V. parahaemolyticus SNPs
DOI10.1016/j.dci.2023.104900
通讯作者Li, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
英文摘要Lysin motif (LysM) is a functional domain that can bind to peptidoglycans, chitin and their derivatives. The LysM-containing proteins participate in multiple biological processes, such as the hydrolysis of bacterial cell walls and the perception of PAMPs in plants and high animals. In the present study, two genes encoding LysM-containing proteins, designated as LvLysM1 and LvLysM2, were identified in the Pacific white shrimp, Litopenaeus vannamei, and their functions during Vibrio infection were analyzed. The open-reading frame (ORF) of LvLysM1 was 795 bp, only encoding a LysM domain at the N-terminal region. The ORF of LvLysM2 was 834 bp, encoding a LysM domain at the central region and a transmembrane region at the C-terminal region. Both LvLysM1 and LvLysM2 were widely transcribed in all tested shrimp tissues. Enzyme-linked immunosorbent assay (ELISA) showed that the recombinant protein of LvLysM2 could bind to different bacterial polysaccharides, while LvLysM1 showed no direct binding activity. The transcripts of LvLysMs in gills increased significantly after infection with Vibrio parahaemolyticus. When LvLysM1 or LvLysM2 was knocked down by dsRNA, the mortality of shrimp was significantly increased after infection with Vibrio parahaemolyticus. Interestingly, some SNPs existed in these two genes were apparently correlated with the VpAHPND resistance of shrimp. These results suggested that LvLysM1 and LvLysM2 might contribute to the disease resistance of shrimp. The data provide new knowledge about the function of LysM-containing proteins in shrimp and potential genetic markers for disease resistance breeding.
WOS关键词MOTIF (LYSM)-CONTAINING PROTEIN ; PEPTIDOGLYCAN-BINDING DOMAIN ; ANTIBACTERIAL RESPONSES ; AEROMONAS-HYDROPHILA ; NECROSIS DISEASE ; SHRIMP ; GENE ; EXPRESSION ; ASSOCIATION ; CLONING
资助项目Key Program of National Natural Science Foundation of China[31972829] ; General Program of National Natural Science Foundation of China ; earmarked fund for CARS-48 ; Taishan Scholars Program ; [31830100]
WOS研究方向Fisheries ; Immunology ; Veterinary Sciences ; Zoology
语种英语
出版者ELSEVIER SCI LTD
WOS记录号WOS:001058518700001
源URL[http://ir.qdio.ac.cn/handle/337002/182873]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Li, Shihao; Li, Fuhua
作者单位1.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
5.Chinese Acad Sci, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Miao, Miao,Li, Shihao,Yu, Yang,et al. LysM-containing proteins function in the resistance of Litopenaeus vannamei against Vibrio parahaemolyticus infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2023,148:9.
APA Miao, Miao,Li, Shihao,Yu, Yang,&Li, Fuhua.(2023).LysM-containing proteins function in the resistance of Litopenaeus vannamei against Vibrio parahaemolyticus infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,148,9.
MLA Miao, Miao,et al."LysM-containing proteins function in the resistance of Litopenaeus vannamei against Vibrio parahaemolyticus infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 148(2023):9.

入库方式： OAI收割

来源：海洋研究所

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