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ACD-containing chaperones reveal the divergent thermo-tolerance in penaeid shrimp

文献类型：期刊论文

作者Zhang, Xiaoxi1,2; Zhang, Xiaojun1,2,3,4; Yuan, Jianbo1,2,3,4; Li, Fuhua1,2,3,4
刊名SCIENCE OF THE TOTAL ENVIRONMENT
出版日期2023-07-01
卷号880页码:12
ISSN号0048-9697
关键词ACD-containing genes Chaperones Divergent thermo-tolerance Penaeid shrimp Gene family expansion Adaptation
DOI10.1016/j.scitotenv.2023.163239
通讯作者Li, Fuhua(fhli@qdio.ac.cn)
英文摘要The a-crystallin domain-containing (ACD-containing) gene family, which includes typical small heat shock proteins (sHSPs), is the most ubiquitous and diverse family of putative chaperones in all organisms, including eukaryotes and prokaryotes. In the present study, approximately 54-117 ACD-containing genes were identified in five penaeid shrimp species, yielding a significant expansion in comparison with other crustaceans (generally 6-20 ACDcontaining genes). Unlike typical sHSPs, which contain a single ACD domain, the ACD-containing genes of penaeid shrimp contain additional ACD domains (3-7 domains, in general), thus having a larger molecular weight and a more complex 3D structure. As indicated by the RNA-seq and qRT-PCR results, the ACD-containing genes of penaeid shrimp showed a strong response to high temperatures. Furthermore, heterologous expression and citrate synthase assays of three representative ACD-containing genes confirmed that their chaperone activity could enhance the thermotolerance of E. coli and prevent the aggregation of substrate proteins at high temperatures. Compared with penaeid shrimp species with a relatively low thermo-tolerance (Fenneropenaeus chinensis and Marsupenaeus japonicus), the species with high thermo-tolerance (Litopenaeus vannamei and Fenneropenaeus indicus) contained more ACD-containing genes due to tandem duplication and exhibited biased expression levels under high temperatures. This can explain the divergent thermo-tolerance of different penaeid shrimp species. In conclusion, the ACD-containing genes in penaeid shrimp could be assigned as new chaperones and contribute to their divergent thermo-tolerance phenotypes and adaptations to the ecological environment.
WOS关键词ALPHA-CRYSTALLIN DOMAIN ; HEAT-SHOCK PROTEINS ; LITOPENAEUS-VANNAMEI ; TEMPERATURE ; PACIFIC ; ALIGNMENT ; STRESS
资助项目National Key Research and Development Program of China[2018YFD0900103] ; National Natural Science Foundation of China[42206091] ; China Postdoctoral Science Foundation[2021M703249] ; Agriculture Research System-48 of China[CARS-48] ; Taishan Scholars Program ; Oceanographic Data Center, IOCAS
WOS研究方向Environmental Sciences & Ecology
语种英语
出版者ELSEVIER
WOS记录号WOS:000985387000001
源URL[http://ir.qdio.ac.cn/handle/337002/182939]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Li, Fuhua
作者单位1.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Xiaoxi,Zhang, Xiaojun,Yuan, Jianbo,et al. ACD-containing chaperones reveal the divergent thermo-tolerance in penaeid shrimp[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2023,880:12.
APA Zhang, Xiaoxi,Zhang, Xiaojun,Yuan, Jianbo,&Li, Fuhua.(2023).ACD-containing chaperones reveal the divergent thermo-tolerance in penaeid shrimp.SCIENCE OF THE TOTAL ENVIRONMENT,880,12.
MLA Zhang, Xiaoxi,et al."ACD-containing chaperones reveal the divergent thermo-tolerance in penaeid shrimp".SCIENCE OF THE TOTAL ENVIRONMENT 880(2023):12.

入库方式： OAI收割

来源：海洋研究所

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