Identification and characterization of the key lethal toxin from jellyfish Cyanea nozakii
文献类型：期刊论文
| 作者 | Li, Rongfeng2,3; Yu, Huahua2,3 ; Li, Aoyu1,2; Yu, Chunlin2,3; Li, Pengcheng2,3
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| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| 出版日期 | 2023-03-01 |
| 卷号 | 230页码:8 |
| ISSN号 | 0141-8130 |
| 关键词 | Jellyfish Venom Toxin Letoxcn Cyanea nozakii |
| DOI | 10.1016/j.ijbiomac.2023.123176 |
| 通讯作者 | Li, Rongfeng(rongfengli@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn) |
| 英文摘要 | Jellyfish Cyanea nozakii venom is a complex mixture of various toxins, most of which are proteinous biological macromolecules and are considered to be responsible for clinical symptoms or even death after a severe sting. Previous transcriptome and proteome analysis identified hundreds of toxins in the venom, including hemolysins, C-type lectin, phospholipase A2, potassium channel inhibitor, metalloprotease, etc. However, it is not clear which toxin in the venom plays the most important role in lethality. Herein, we isolated the key lethal toxin (Letoxcn) from jellyfish Cyanea nozakii using anion exchange chromatography, size-exclusion chromatography, and cation exchange chromatography. The molecular weight of Letoxcn is similar to 50 kDa with the N-terminal sequences of QADAEKVNLPVGVCV. Peptide mass fingerprinting analysis of Letoxcn shows that it may have some motifs of phospholipase, metalloproteinase, thrombin-like enzyme, potassium channel toxin, etc. However, only metalloproteinase activity but no hemolytic, PLA2, or blood coagulation activity was observed from in vitro toxicity analysis. Overall, this study uncovered and characterized the key lethal toxin in the venom of jellyfish Cyanea nozakii, which will not only help to reveal the molecule mechanism of the lethality, but also develop effective treatment like antivenom for this jellyfish sting in the future. |
| WOS关键词 | BOX JELLYFISH ; PARTIAL-PURIFICATION ; CHIRONEX-FLECKERI ; SEA WASP ; VENOM PROTEINS ; STINGS ; NEUROTOXIN ; MANAGEMENT ; HEMOLYSIN ; TOXICITY |
| 资助项目 | National Natural Science Founda-tion of China[41876164] ; Natural Science Foundation of Shandong Province[ZR2019QD012] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000990027600001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/183002]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Rongfeng; Li, Pengcheng |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Rongfeng,Yu, Huahua,Li, Aoyu,et al. Identification and characterization of the key lethal toxin from jellyfish Cyanea nozakii[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2023,230:8. |
| APA | Li, Rongfeng,Yu, Huahua,Li, Aoyu,Yu, Chunlin,&Li, Pengcheng.(2023).Identification and characterization of the key lethal toxin from jellyfish Cyanea nozakii.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,230,8. |
| MLA | Li, Rongfeng,et al."Identification and characterization of the key lethal toxin from jellyfish Cyanea nozakii".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 230(2023):8. |
入库方式： OAI收割
来源：海洋研究所
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