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Effects of different dehydration methods on the preservation of aortic and renal glycocalyx structures in mice

文献类型：期刊论文

作者Li, Zhi1,4,5; Zhang, Quanbin1,5; Sun, Yuan-yuan1; Wu, Ning1,2,3,5
刊名HELIYON
出版日期2023-04-01
卷号9期号:4页码:9
关键词Glycocalyx Dehydration Transmission electron microscopy
DOI10.1016/j.heliyon.2023.e15197
通讯作者Sun, Yuan-yuan(sunyuanyuan@qdio.ac.cn) ; Wu, Ning(wuning@qdio.ac.cn)
英文摘要Glycocalyx is located outside the vascular endothelial cells playing an important role in vascular homeostasis. However, lacking efficient detection methods is one of the biggest obstacles to study the glycocalyx. In this study, three dehydration methods were used to compare the preservation of HUVEC, aorta and kidney glycocalyx by transmission electron microscope. The chemical pre -fixation was performed by lanthanum nitrate staining, and the mice aorta and renal glycocalyx were prepared by different dehydration methods such as ethanol gradient, acetone gradient and low temperature dehydration. HUVEC glycocalyx was prepared by acetone gradient and low temperature dehydration. Low temperature dehydration method preserves HUVEC and mice aortic glycocalyx completely, which had a certain thickness and presented a needle-like structure. But for mice kidney, the acetone gradient dehydration preparation method could better preserve the glycocalyx integrity than other two methods. In conclusion, low temperature dehydration method is suitable for HUVEC and aortic glycocalyx preservation, acetone gradient dehydration method is more suitable for kidney glycocalyx preservation.
WOS关键词ENDOTHELIAL GLYCOCALYX ; DYSFUNCTION ; CELL ; SULFATE ; BIOLOGY ; LAYER
资助项目Innovative Research Group Project of the National Natural Science Foundation of China[42176137] ; Nantong Science and Technology Project, China[MS12021037]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000999832300001
出版者CELL PRESS
源URL[http://ir.qdio.ac.cn/handle/337002/183165]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Sun, Yuan-yuan; Wu, Ning
作者单位1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
3.Nantong Zhongke Marine Sci & Technol Res & Dev Ctr, Nantong, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Qingdao Natl Lab Marine Sci & Technol, Dept Drugs & Bioprod, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Li, Zhi,Zhang, Quanbin,Sun, Yuan-yuan,et al. Effects of different dehydration methods on the preservation of aortic and renal glycocalyx structures in mice[J]. HELIYON,2023,9(4):9.
APA Li, Zhi,Zhang, Quanbin,Sun, Yuan-yuan,&Wu, Ning.(2023).Effects of different dehydration methods on the preservation of aortic and renal glycocalyx structures in mice.HELIYON,9(4),9.
MLA Li, Zhi,et al."Effects of different dehydration methods on the preservation of aortic and renal glycocalyx structures in mice".HELIYON 9.4(2023):9.

入库方式： OAI收割

来源：海洋研究所

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