High-throughput prediction and characterization of antimicrobial peptides from multi-omics datasets of Chinese tubular cone snail (Conus betulinus)
文献类型：期刊论文
| 作者 | Li, Ruihan6,7; Huang, Yu6; Peng, Chao6; Gao, Zijian8; Liu, Jie2; Yin, Xiaoting1,4,9 ; Gao, Bingmiao3; Ovchinnikova, Tatiana V.5; Qiu, Limei1,9; Bian, Chao6,7
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| 刊名 | FRONTIERS IN MARINE SCIENCE
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| 出版日期 | 2022-12-23 |
| 卷号 | 9页码:13 |
| 关键词 | Conus betulinus antimicrobial peptide multi-omics in silico prediction in vitro assessment |
| DOI | 10.3389/fmars.2022.1092731 |
| 通讯作者 | Qiu, Limei(shiqiong@genomics.cn) ; Bian, Chao(bianchao@genomics.cn) ; Shi, Qiong(qiulimei@qdio.ac.cn) |
| 英文摘要 | Individual cone snail (Conus sp.) contains thousands of bioactive peptides, but there are limited studies on its antimicrobial peptides (AMPs). Here, we investigated AMPs along with AMP-derived genes in the representative Chinese tubular cone snail (C. betulinus) by integration of our previously published multi-omics (genomics, transcriptomics, and peptidomics) data. We identified a total of 466 putative AMP-derived genes from the genome, and most of them were annotated as histones. While at the mRNA level, only 77 AMP-derived genes were confirmed with transcriptomic evidence, among them Ubiquicidin, cgUbiquitin, Ap, and VK10 were the most abundant transcripts in the venom gland. In addition, 30 AMPs were further validated by peptidomics data. After in silico analysis including 3D modeling, 11 putative AMPs were chemically synthesized for an in vitro assessment, and eight peptides were proved with good antifungal activity. In summary, this work systematically characterized the AMP repertoire in C. betulinus. This valuable genetic resource provides a solid foundation for in-depth therapeutic applications and drug development. |
| WOS关键词 | PACIFIC OYSTER ; PURIFICATION ; CONOTOXINS ; HISTONES ; GENOMES |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000908042500001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/183272]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Qiu, Limei; Bian, Chao; Shi, Qiong |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.BGI Shenzhen, BGI Genom, Shenzhen, Peoples R China 3.Hainan Med Univ, Sch Pharm, Key Lab Trop Translat Med,Minist Educ, Hainan Prov Key Lab Res & Dev Herbs, Haikou, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China 5.Russian Acad Sci, MM Shemyakin & Yu A Ovchinnikov Inst Bioorgan Chem, Moscow, Russia 6.BGI Acad Marine Sci, Shenzhen Key Lab Marine Genom, Guangdong Prov Key Lab Mol Breeding Marine Econ An, BGI Marine, Shenzhen, Peoples R China 7.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 8.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi, Peoples R China 9.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Ruihan,Huang, Yu,Peng, Chao,et al. High-throughput prediction and characterization of antimicrobial peptides from multi-omics datasets of Chinese tubular cone snail (Conus betulinus)[J]. FRONTIERS IN MARINE SCIENCE,2022,9:13. |
| APA | Li, Ruihan.,Huang, Yu.,Peng, Chao.,Gao, Zijian.,Liu, Jie.,...&Shi, Qiong.(2022).High-throughput prediction and characterization of antimicrobial peptides from multi-omics datasets of Chinese tubular cone snail (Conus betulinus).FRONTIERS IN MARINE SCIENCE,9,13. |
| MLA | Li, Ruihan,et al."High-throughput prediction and characterization of antimicrobial peptides from multi-omics datasets of Chinese tubular cone snail (Conus betulinus)".FRONTIERS IN MARINE SCIENCE 9(2022):13. |
入库方式： OAI收割
来源：海洋研究所
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