中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 海洋研究所
  3. 中国科学院海洋研究所
  4. 实验海洋生物学重点实验室
Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus)

文献类型：期刊论文

作者Qi, Ting3; Wu, Lele3; Yu, Jiachen1; Song, Zongcheng2; Liu, Feng4; Li, Jun4; Song, Xiefa3; Li, Xian3
刊名SCIENCE OF THE TOTAL ENVIRONMENT
出版日期2023-02-25
卷号861页码:12
ISSN号0048-9697
关键词Phosphate Toxicity Metabolomics Histopathological damage Gene expression
DOI10.1016/j.scitotenv.2022.160430
通讯作者Li, Xian(lixian@ouc.edu.cn)
英文摘要Phosphate, as the main nutrient factor of lake eutrophication brought by pollutants discharged from agriculture and industry, is always considered to be a low-toxicity substance to aquatic animals. But the toxicity mechanism is unclear, and published information is limited. In this study, a 96 h acute stress experiment was conducted on juvenile turbot (Scophthalmus maximus) with 0, 10, and 60 mg/L phosphate solutions. Metabonomic analysis revealed that low -dose phosphate (10 mg/L) disrupted glycerophospholipid, purine, and glycolipid metabolism, as well as the tricarbox-ylic acid (TCA) cycle in juveniles, even at 96 h of stress, which may lead to cell structure damage and signal recognition disorder between cells. Upregulated key genes in the main glycerophospholipid metabolic pathways, which matched the results of the metabolomic study, were detected. Furthermore, low-dose phosphate (10 mg/L) induced oxidative stress and immunotoxicity in fish, resulting in the raising of relevant genes expression such as cat and sod in liver and kidney. In addition, all phosphate-treated groups had induced lesions on gill tissue, as evidenced by pathological observations. In this study on toxic effects on and mechanism of phosphate in aquatic animals using metabolomics, gene expression, and histopathology, we confirm that acute low-dose phosphate could disrupt glycerophospholipid metabolism and induce stress in juvenile turbot. This can provide advice on the amount of phosphate accumulation for marine fish farming and on protecting species diversity and marine ecosystem from the point of view of phosphate toxicity to marine animals.
WOS关键词ACTIVATED PROTEIN-KINASE ; IMMUNE-RESPONSES ; STOCKING DENSITY ; CELLULAR-ENERGY ; MYOSIN-II ; WATER ; HEALTH ; EUTROPHICATION ; PERFORMANCE ; EXPRESSION
资助项目National Natural Science Foundation of China[42061160365] ; National Natural Science Foundation of China[41976122] ; Young Talent Program of Ocean University of China[862201013156] ; Science and Technology Development Planning Projects of Huancui ; China Agriculture Research System[CARS -47]
WOS研究方向Environmental Sciences & Ecology
语种英语
出版者ELSEVIER
WOS记录号WOS:000901627600013
源URL[http://ir.qdio.ac.cn/handle/337002/183281]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Li, Xian
作者单位1.Jiangsu Ocean Univ, Sch Marine Sci & Fisheries, Lianyungang 222005, Jiangsu, Peoples R China
2.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China
3.Ocean Univ China, Fisheries Coll, Key Lab Mariculture, Minist Educ, Qingdao 266001, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Qi, Ting,Wu, Lele,Yu, Jiachen,et al. Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus)[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2023,861:12.
APA Qi, Ting.,Wu, Lele.,Yu, Jiachen.,Song, Zongcheng.,Liu, Feng.,...&Li, Xian.(2023).Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus).SCIENCE OF THE TOTAL ENVIRONMENT,861,12.
MLA Qi, Ting,et al."Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus)".SCIENCE OF THE TOTAL ENVIRONMENT 861(2023):12.

入库方式： OAI收割

来源：海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。