Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction
文献类型：期刊论文
| 作者 | Zhou, Yanmei1,5,6; Xie, Shupu5; Liu, Bo5; Wang, Cong5; Huang, Yibo5; Zhang, Xiaoru2,5; Zhang, Shusheng3,4 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2023-01-30 |
| 页码 | 8 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.2c04484 |
| 通讯作者 | Zhang, Xiaoru(zhangxr7407@126.com) ; Zhang, Shusheng(shushzhang@126.com) |
| 英文摘要 | Herein, a chemiluminescence (CL) biosensor based on CRISPR-Cas12a and cation exchange reaction was constructed to detect the biomarker microRNA-21 (miRNA-21). The rolling circle amplification (RCA) reaction was introduced to convert each target RNA strand into a long single-stranded DNA with repeated sequences, which acted as triggers to initiate the transcleavage activity of CRISPR-Cas12a. The activated Cas12a could cleave the biotinylated linker DNA of CuS nanoparticles (NPs) to inhibit the binding of CuS NPs to streptavidin immobilized on the surface of the microplate, which strongly reduced the generation of Cu2+ from a cation exchange between CuS NPs and AgNO3, and thus efficiently suppressed the CL of Cu2+-luminol-H2O2 system, giving a "signal off" biosensor. With the multiple amplification, the detection limit of the developed sensor for miRNA-21 reached 16 aM. In addition, this biosensor is not only suitable for a professional chemiluminescence instrument but also for a smartphone used as a detection tool for the purpose of portable and low-cost assay. This method could be used to specifically detect quite a low level of miRNA-21 in human serum samples and various cancer cells, indicating its potential in ultrasensitive molecular diagnostics. |
| WOS关键词 | MICRORNA EXPRESSION ; BIOLOGICAL CONSTITUENT ; AMPLIFICATION |
| 资助项目 | National Natural Science Foundation of China[22276103] ; National Natural Science Foundation of China[22274068] ; National Natural Science Foundation of China[22076073] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000925401700001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/183449]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Zhang, Xiaoru; Zhang, Shusheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Univ Sci & Technol, Key Lab Opt Elect Sensing & Analyt Chem Life Sci, Shandong Key Lab Biochem Anal, MOE, Qingdao 266042, Peoples R China 3.Equipment & Diag Therapy Integrat Univ Shandong, Linyi Univ, Coll Chem & Chem Engn, Collaborat Innovat Ctr Tumor Marker Detect Technol, Linyi 276000, Peoples R China 4.Linyi Univ, Coll Chem & Chem Engn, Linyi 276000, Peoples R China 5.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Peoples R China 6.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Yanmei,Xie, Shupu,Liu, Bo,et al. Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction[J]. ANALYTICAL CHEMISTRY,2023:8. |
| APA | Zhou, Yanmei.,Xie, Shupu.,Liu, Bo.,Wang, Cong.,Huang, Yibo.,...&Zhang, Shusheng.(2023).Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction.ANALYTICAL CHEMISTRY,8. |
| MLA | Zhou, Yanmei,et al."Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction".ANALYTICAL CHEMISTRY (2023):8. |
入库方式： OAI收割
来源：海洋研究所
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