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The Diversity and Metabolism of Culturable Nitrate-Reducing Bacteria from the Photic Zone of the Western North Pacific Ocean

文献类型：期刊论文

作者Jiang, Zhichen1,2,5; Liu, Sizhen3,4; Zhang, Dechao1,2,5; Sha, Zhongli1,2,5
刊名MICROBIAL ECOLOGY
出版日期2023-08-08
页码9
ISSN号0095-3628
关键词Diversity Nitrate-reducing bacteria The photic zone Pacific Ocean
DOI10.1007/s00248-023-02284-w
通讯作者Zhang, Dechao(zhangdechao@qdio.ac.cn)
英文摘要To better understand bacterial communities and metabolism under nitrogen deficiency, 154 seawater samples were obtained from 5 to 200 m at 22 stations in the photic zone of the Western North Pacific Ocean. Total 634 nitrate-utilizing bacteria were isolated using selective media and culture-dependent methods, and 295 of them were positive for nitrate reduction. These nitrate-reducing bacteria belonged to 19 genera and 29 species and among them, Qipengyuania flava, Roseibium aggregatum, Erythrobacter aureus, Vibrio campbellii, and Stappia indica were identified from all tested seawater layers of the photic zone and at almost all stations. Twenty-nine nitrate-reducing strains representing different species were selected for further the study of nitrogen, sulfur, and carbon metabolism. All 29 nitrate-reducing isolates contained genes encoding dissimilatory nitrate reduction or assimilatory nitrate reduction. Six nitrate-reducing isolates can oxidize thiosulfate based on genomic analysis and activity testing, indicating that nitrate-reducing thiosulfate-oxidizing bacteria exist in the photic zone. Five nitrate-reducing isolates obtained near the chlorophyll a-maximum layer contained a dimethylsulfoniopropionate synthesis gene and three of them contained both dimethylsulfoniopropionate synthesis and cleavage genes. This suggests that nitrate-reducing isolates may participate in dimethylsulfoniopropionate synthesis and catabolism in photic seawater. The presence of multiple genes for chitin degradation and extracellular peptidases may indicate that almost all nitrate-reducing isolates (28/29) can use chitin and proteinaceous compounds as important sources of carbon and nitrogen. Collectively, these results reveal culturable nitrate-reducing bacterial diversity and have implications for understanding the role of such strains in the ecology and biogeochemical cycles of nitrogen, sulfur, and carbon in the oligotrophic marine photic zone.
WOS关键词NITROGEN-CYCLE ; SP. NOV. ; MARINE ; COMMUNITIES ; REDUCTION ; GENE ; SEA ; REDUCTASES ; ABUNDANCES ; STAPPIA
资助项目Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050302] ; National Natural Science Foundation of China[41976166] ; National Natural Science Foundation of China[42025603] ; Marine Samp;T Fund of Shandong Province for Qingdao Marine Science and Technology enter[2022QNLM030004-3] ; Marine Samp;T Fund of Shandong Province for Qingdao Marine Science and Technology enter[LSKJ202203100] ; NSFC Shiptime Sharing Project[42249901]
WOS研究方向Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology
语种英语
出版者SPRINGER
WOS记录号WOS:001044333900001
源URL[http://ir.qdio.ac.cn/handle/337002/182717]  
专题海洋研究所_海洋生物分类与系统演化实验室
通讯作者Zhang, Dechao
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao Key Lab Marine Biodivers & Conservat, Qingdao 266071, Peoples R China
3.Huazhong Agr Univ, Coll Informat, Hubei Key Lab Agr Bioinformat, Wuhan 430070, Peoples R China
4.Huazhong Agr Univ, Natl Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
5.Laoshan Lab, Qingdao 266237, Peoples R China
推荐引用方式
GB/T 7714		 Jiang, Zhichen,Liu, Sizhen,Zhang, Dechao,et al. The Diversity and Metabolism of Culturable Nitrate-Reducing Bacteria from the Photic Zone of the Western North Pacific Ocean[J]. MICROBIAL ECOLOGY,2023:9.
APA Jiang, Zhichen,Liu, Sizhen,Zhang, Dechao,&Sha, Zhongli.(2023).The Diversity and Metabolism of Culturable Nitrate-Reducing Bacteria from the Photic Zone of the Western North Pacific Ocean.MICROBIAL ECOLOGY,9.
MLA Jiang, Zhichen,et al."The Diversity and Metabolism of Culturable Nitrate-Reducing Bacteria from the Photic Zone of the Western North Pacific Ocean".MICROBIAL ECOLOGY (2023):9.

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来源：海洋研究所

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