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Next-generation sequencing and metabarcoding to understand the ecology of benthic foraminiferal community in the Bering Sea

文献类型：期刊论文

作者Li, Haotian3,6; Lei, Yanli2,3,5,6; Li, Tiegang1,5; Saraswat, Rajeev4
刊名JOURNAL OF SEA RESEARCH
出版日期2023-02-01
卷号191页码:11
ISSN号1385-1101
关键词The Bering Sea Benthic foraminifera Molecular diversity Community composition Next -generation sequencing
DOI10.1016/j.seares.2022.102321
通讯作者Lei, Yanli(leiyanli@qdio.ac.cn) ; Li, Tiegang(tgli@fio.org.cn)
英文摘要The sedimentary archives from the Bering Sea can provide excellent records of the global climate shift. However, paleoclimatic studies from the Bering Sea are limited due to the lack of well calibrated proxies. Various char-acteristics of foraminifera are frequently used as paleoclimatic and paleoceanographic proxies. A precise un-derstanding of the ecology of foraminiferal species is required to use it as proxy. Here, we use next-generation sequencing and metabarcoding to understand the ecology of benthic foraminiferal community in the Bering Sea. The total genomic deoxyribonucleic acid (DNA) was extracted from 11 surface sediment samples collected from the Bering Sea. The foraminiferal small sub unit (SSU) rDNA was amplified with foraminiferal-specific primers. A total of 2,170,762 effective tags and 1046 operational taxonomic unit (OTUs) were obtained after next -generation sequencing, strict quality control, and data processing. The results showed that the station BR07 had the highest number of OTUs. The highest foraminiferal Margalef index and Shannon-Wiener index were obtained at BR06. Rotaliida accounted for the highest proportion, followed by Textulariida and Monothalamida, with the lowest proportion of Miliolida. From the principal component analysis (PCA), Leptohalysis scottii, Stainforthiidae_X sp., and Rectuvigerina phlegeri were found to be the more important species in the Bering Sea. Based on the redundancy analysis (RDA) and Spearman correlation analysis, we found that the grain size and water depth exhibited significant control on foraminiferal community. The foraminiferal reads were positively correlated with the grain size, whereas the Shannon-Wiener index was negatively correlated with it. The water depth was positively correlated with OTUs, Leptohalysis scottii, and Rectuvigerina phlegeri. The clustering analysis showed that Bering Sea stations cluster together with Yellow Sea stations of similar water depth. The water depth was a bigger factor for foraminiferal community than the geographical distance.
WOS关键词PALEOCEANOGRAPHIC CHANGES ; SURFACE SEDIMENTS ; BAFFIN-BAY ; DIVERSITY ; TEMPERATURE ; PACIFIC ; IMPACT ; ICE ; BIODIVERSITY ; ASSEMBLAGES
资助项目Laoshan Laboratory Science and technology innovation project[LSKJ202204200] ; National Science Foundation of China[41976058] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; Wenhai Program of Qingdao Pilot National Laboratory of Marine Science and Technology[2021WHZZB0804]
WOS研究方向Marine & Freshwater Biology ; Oceanography
语种英语
出版者ELSEVIER
WOS记录号WOS:000918742000001
源URL[http://ir.qdio.ac.cn/handle/337002/183355]  
专题海洋研究所_海洋生物分类与系统演化实验室
通讯作者Lei, Yanli; Li, Tiegang
作者单位1.Minist Nat Resources MNR, Inst Oceanog 1, Key Lab Marine Sedimentol & Environm Geol, Qingdao 266061, Peoples R China
2.Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519082, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Lab Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China
4.CSIR Natl Inst Oceanog, Geol Oceanog Div, Panaji 403004, Goa, India
5.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Li, Haotian,Lei, Yanli,Li, Tiegang,et al. Next-generation sequencing and metabarcoding to understand the ecology of benthic foraminiferal community in the Bering Sea[J]. JOURNAL OF SEA RESEARCH,2023,191:11.
APA Li, Haotian,Lei, Yanli,Li, Tiegang,&Saraswat, Rajeev.(2023).Next-generation sequencing and metabarcoding to understand the ecology of benthic foraminiferal community in the Bering Sea.JOURNAL OF SEA RESEARCH,191,11.
MLA Li, Haotian,et al."Next-generation sequencing and metabarcoding to understand the ecology of benthic foraminiferal community in the Bering Sea".JOURNAL OF SEA RESEARCH 191(2023):11.

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来源：海洋研究所

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