Photoreversible DNA nanoswitch-based eluent-free strategy for the direct and effective isolation of highly-active thrombin from whole blood
文献类型：期刊论文
| 作者 | Lin, Yanna1; Xu, Yulong1; Xing, Yanzhi1; Liu, Nazhen2; Chen, Xuwei1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2023-06-01 |
| 卷号 | 239页码:11 |
| ISSN号 | 0141-8130 |
| 关键词 | Size charge screening Photoresponse DNA nanoswitch Eluent-free Thrombin |
| DOI | 10.1016/j.ijbiomac.2023.124359 |
| 通讯作者 | Liu, Nazhen(liunazhen@qdio.ac.cn) ; Chen, Xuwei(chenxuwei@mail.neu.edu.cn) |
| 英文摘要 | This study proposes an eluent-free isolation strategy for the direct isolation of thrombin from whole blood via tandem temperature/pH dual-responsive polyether sulfone monolith and photoreversible DNA nanoswitchfunctionalized metal-organic framework (MOF) aerogel. Temperature/pH dual-responsive microgel immobilized on polyether sulfone monolith was adopted to remove the matrix complexity of blood sample via size/ charge screening effect. Photoreversible DNA nanoswitches, comprising thrombin aptamer, aptamer complementary ssDNA (cDNA) and the azobenzene-modified ssDNA (control DNA), were functionalized on MOF aerogel to offer efficient capturing of thrombin under irradiation of ultraviolet light (365 nm), driven by electrostatic and hydrogen bond interactions. The release of captured thrombin was easily achieved by changing the complementary behaviors of DNA strands via blue light (450 nm) irradiation. Thrombin with purity higher than 95 % can be directly obtained from whole blood using this tandem isolation procedure. Fibrin production and substrate chromogenic tests showed that the released thrombin possessed high biological activity. The photoreversible thrombin capturing-release strategy is merited with eluent-free, avoiding the loss of activity of thrombin in chemical circumstances and undesired dilution, providing a robust guarantee for subsequent application. |
| WOS关键词 | REVERSIBLE REGULATION ; PROTEIN INTERACTIONS ; G-QUADRUPLEX ; DEPLETION ; ABUNDANT ; APTAMER ; ADSORPTION ; SEPARATION ; STABILITY |
| 资助项目 | LiaoNing Science and Technology Development Foundation Guided by Central Government[2022JH6/100100024] ; Fundamental Research Funds for the Cen-tral Universities[N2005027] ; National Natural Science Foundation of China[22074011] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000983807400001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/183008]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 通讯作者 | Liu, Nazhen; Chen, Xuwei |
| 作者单位 | 1.Northeastern Univ, Coll Sci, Res Ctr Analyt Sci, Dept Chem, Box 332, Shenyang 110819, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Yanna,Xu, Yulong,Xing, Yanzhi,et al. Photoreversible DNA nanoswitch-based eluent-free strategy for the direct and effective isolation of highly-active thrombin from whole blood[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2023,239:11. |
| APA | Lin, Yanna,Xu, Yulong,Xing, Yanzhi,Liu, Nazhen,&Chen, Xuwei.(2023).Photoreversible DNA nanoswitch-based eluent-free strategy for the direct and effective isolation of highly-active thrombin from whole blood.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,239,11. |
| MLA | Lin, Yanna,et al."Photoreversible DNA nanoswitch-based eluent-free strategy for the direct and effective isolation of highly-active thrombin from whole blood".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 239(2023):11. |
入库方式： OAI收割
来源：海洋研究所
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