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  4. 新药研究国家重点实验室
Forsythia suspensa (Thunb.) Vahl extract ameliorates ulcerative colitis via inhibiting NLRP3 inflammasome activation through the TLR4/MyD88/NF-κB pathway

文献类型：期刊论文

作者Tong, Xiao8,9; Chen, Li8; He, Shijun7; Liu, Shuangshuang9; Yao, Jiaying6; Shao, Zhenguang5,9; Ye, Yang4,6,9; Yao, Sheng3,6,9; Lin, Zemin1,8; Zuo, Jianping2,8,9
刊名IMMUNITY INFLAMMATION AND DISEASE
出版日期2023-11-01
卷号11期号:11页码:14
关键词macrophages NLRP3 inflammasome phillygenin ulcerative colitis
DOI10.1002/iid3.1069
通讯作者He, Shijun(heshijun@shutcm.edu.cn) ; Yao, Sheng(yaosheng@simm.ac.cn) ; Lin, Zemin(linzemin@simm.ac.cn) ; Zuo, Jianping(jpzuo@simm.ac.cn)
英文摘要Background Ulcerative colitis (UC), a chronic inflammatory disease, is caused by abnormal immune system reactions resulting in inflammation and ulcers in the large intestine. Phillygenin (PHI) is a natural compound found in Forsythia suspensa (Thunb.) Vahl, which is known for its antipyretic, anti-inflammatory, antiobesity, and other biological activities. However, the therapeutic role and molecular mechanisms of PHI on UC are still insufficiently researched.Methods In this study, dextran sulfate sodium (DSS) and 2.5% 2,4,6-trinitro-Benzenesulfonic acid (TNBS)-induced acute UC were used to investigate the therapeutic effects of PHI. We evaluated the effects of PHI on disease activity index (DAI), body weight, mortality, intestinal mucosal barrier, cytokine secretion, and macrophage infiltration into colon tissue using various techniques such as flow cytometry, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), RT-qPCR, and Western blot analysis.Results Our findings revealed that PHI has therapeutic properties in UC treatment. PHI was able to maintain body weight, reduce DAI and mortality, restore the intestinal mucosal barrier, and inhibit cytokine secretion. Flow cytometry assay and immunofluorescence indicated that PHI reduces macrophage infiltration into colon tissue. Mechanistically, PHI may exert anti-inflammatory effects by downregulating the TLR4/MyD88/NF-kappa B pathway and inhibiting the activation of NLRP3 inflammasome.Conclusion In conclusion, PHI possesses significant anti-inflammatory properties and is expected to be a potential drug for UC treatment. Our study delves into the underlying mechanisms of PHI therapy and highlights the potential for further research in developing PHI-based treatments for UC.
资助项目We are thankful to financial support from the National Natural Science Foundation of China (82374108, 81903485, 81871240, and 21920102003), Shanghai Municipal Science and Technology Major Project, and the Key-Area Research and Development Program of Guangd[82374108] ; We are thankful to financial support from the National Natural Science Foundation of China (82374108, 81903485, 81871240, and 21920102003), Shanghai Municipal Science and Technology Major Project, and the Key-Area Research and Development Program of Guangd[81903485] ; We are thankful to financial support from the National Natural Science Foundation of China (82374108, 81903485, 81871240, and 21920102003), Shanghai Municipal Science and Technology Major Project, and the Key-Area Research and Development Program of Guangd[81871240] ; We are thankful to financial support from the National Natural Science Foundation of China (82374108, 81903485, 81871240, and 21920102003), Shanghai Municipal Science and Technology Major Project, and the Key-Area Research and Development Program of Guangd[21920102003] ; National Natural Science Foundation of China ; Shanghai Municipal Science and Technology Major Project[2020B0303070002] ; Key-Area Research and Development Program of Guangdong Province
WOS研究方向Immunology
语种英语
WOS记录号WOS:001099625500001
出版者WILEY
源URL[http://119.78.100.183/handle/2S10ELR8/307875]  
专题新药研究国家重点实验室
通讯作者He, Shijun; Yao, Sheng; Lin, Zemin; Zuo, Jianping
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
2.Shanghai Univ Tradit Chinese Med, Lab Immunol & Virol, Shanghai, Peoples R China
3.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Zhongshan, Peoples R China
4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
5.Chinese Acad Sci, Res Ctr, State Key Lab Drug Res & Nat Prod, Shanghai, Peoples R China
6.Jiangxi Univ Tradit Chinese Med, Coll Pharm, Nanchang, Peoples R China
7.Shanghai Univ Tradit Chinese Med, Innovat Res Inst Tradit Chinese Med, Shanghai 201203, Peoples R China
8.Chinese Acad Sci, State Key Lab Drug Res, Shanghai, Peoples R China
9.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Tong, Xiao,Chen, Li,He, Shijun,et al. Forsythia suspensa (Thunb.) Vahl extract ameliorates ulcerative colitis via inhibiting NLRP3 inflammasome activation through the TLR4/MyD88/NF-κB pathway[J]. IMMUNITY INFLAMMATION AND DISEASE,2023,11(11):14.
APA Tong, Xiao.,Chen, Li.,He, Shijun.,Liu, Shuangshuang.,Yao, Jiaying.,...&Zuo, Jianping.(2023).Forsythia suspensa (Thunb.) Vahl extract ameliorates ulcerative colitis via inhibiting NLRP3 inflammasome activation through the TLR4/MyD88/NF-κB pathway.IMMUNITY INFLAMMATION AND DISEASE,11(11),14.
MLA Tong, Xiao,et al."Forsythia suspensa (Thunb.) Vahl extract ameliorates ulcerative colitis via inhibiting NLRP3 inflammasome activation through the TLR4/MyD88/NF-κB pathway".IMMUNITY INFLAMMATION AND DISEASE 11.11(2023):14.

入库方式： OAI收割

来源：上海药物研究所

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