Metagenomic 16S rDNA reads of in situ preserved samples revealed microbial communities in the Yongle blue hole
文献类型：期刊论文
| 作者 | Zhang, Hongxi3,4 ; Wei, Taoshu4; Li, Qingmei4; Fu, Liang2; He, Lisheng4; Wang, Yong1
|
| 刊名 | PEERJ
 |
| 出版日期 | 2023-11-03 |
| 卷号 | 11页码:18 |
| ISSN号 | 2167-8359 |
| 关键词 | Yongle blue hole In situ sampling ISMIFF 16S rRNA gene Anoxic water |
| DOI | 10.7717/peerj.16257 |
| 通讯作者 | Wang, Yong(wangyong@sz.tsinghua.edu.cn) |
| 英文摘要 | Our knowledge on biogeochemistry and microbial ecology of marine blue holes is limited due to challenges in collecting multilayered water column and oxycline zones. In this study, we collected samples from 16 water layers in Yongle blue hole (YBH) located in the South China Sea using the in situ microbial filtration and fixation (ISMIFF) apparatus. The microbial communities based on 16S rRNA metagenomic reads for the ISMIFF samples showed high microbial diversity and consistency among samples with similar dissolved oxygen levels. At the same depth of the anoxic layer, the ISMIFF samples were dominated by sulfate-reducing bacteria from Desulfatiglandales (17.96%). The sulfide concentration is the most significant factor that drives the division of microbial communities in YBH, which might support the prevalence of sulfate-reducing microorganisms in the anoxic layers. Our results are different from the microbial community structures of a Niskin sample of this study and the reported samples collected in 2017, in which a high relative abundance of Alteromonadales (26.59%) and Thiomicrospirales (38.13%), and Arcobacteraceae (11.74%) was identified. We therefore demonstrate a new profile of microbial communities in YBH probably due to the effect of sampling and molecular biological methods, which provides new possibilities for further understanding of the material circulation mechanism of blue holes and expanding anoxic marine water zones under global warming. |
| WOS关键词 | OXYGEN ; WATER ; NITRATE |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | PEERJ INC |
| WOS记录号 | WOS:001101101800003 |
| 源URL | [http://ir.idsse.ac.cn/handle/183446/10667]  |
| 专题 | 深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组 专家咨询委员会 |
| 通讯作者 | Wang, Yong |
| 作者单位 | 1.Tsinghua Univ, Inst Ocean Engn, Shenzhen Int Grad Sch, Shenzhen, Peoples R China 2.Sansha Trackline Inst Coral Reef Environm Protect, Sansha, Hainan, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Hainan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Hongxi,Wei, Taoshu,Li, Qingmei,et al. Metagenomic 16S rDNA reads of in situ preserved samples revealed microbial communities in the Yongle blue hole[J]. PEERJ,2023,11:18. |
| APA | Zhang, Hongxi,Wei, Taoshu,Li, Qingmei,Fu, Liang,He, Lisheng,&Wang, Yong.(2023).Metagenomic 16S rDNA reads of in situ preserved samples revealed microbial communities in the Yongle blue hole.PEERJ,11,18. |
| MLA | Zhang, Hongxi,et al."Metagenomic 16S rDNA reads of in situ preserved samples revealed microbial communities in the Yongle blue hole".PEERJ 11(2023):18. |
入库方式： OAI收割
来源：深海科学与工程研究所
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